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5' Cap-Isolated RNA Pol II Transcripts and ENCODE Tiling Arrays to Identify Gene Expression Changes in Hepatitis C Liver

(Submitter supplied) Recent studies of the human transcriptome suggest that many transcripts have short or lack 3' poly(A) ends. In addition, the length of 3' poly(A) ends may vary for some transcript to regulate mRNA translation. Heterogeneous lengths of poly(A) ends may introduce variations in measuring RNA transcripts using current approaches. We hypothesized that affinity purifying Pol II RNAs by their unique 5' m7GpppN cap regardless of their polyadenylation status would add additional information to genomic expression analyses and possibly overcome the variation in signals sometimes observed with analysis of poly(A) selected mRNA. more...
Organism:
Homo sapiens
Type:
Expression profiling by genome tiling array
Platform:
GPL4127
8 Samples
Download data: TXT
Series
Accession:
GSE18904
ID:
200018904

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