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RNA co-immunoprecipitations with yeast She proteins

(Submitter supplied) RNA co-immunoprecipitations with C-terminal protein A-tagged She proteins. One liter of cells were cultured at 30 degrees in YPAD medium and collected during exponential growth by centrifugation. Cells were washed twice and broken mechanically with glass beads. Extracts were incubated with IgG-agarose beads (Sigma). The beads were washed four times, and She proteins were released from the beads by cleavage with TEV-protease (Invitrogen). more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platforms:
GPL3269 GPL3270
10 Samples
Download data
Series
Accession:
GSE3879
ID:
200003879

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