GEO DataSets

(Submitter supplied) Testis were obtained from 2 sibling pairs of of young male mice, 1 knock-out and 1 heterozygous for the Sirt1 allele, and used to compare global gene expression using spotted oligo microarrays (the MEEBO arrays from the Stanford microarray core- MOE series). Eighty genes were up or down regulated, where the criteria for differential expression was a greater than 2 fold change in expression level on at least 5 of the 6 sample arrays. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5467

6 Samples

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(Submitter supplied) In the context of male reproductive health, epidemiological studies have observed reduced testis size and abnormal sperm counts and morphology in adult men exposed in utero, although these findings are not always repeated. The ambiguity of these reports is confounded by a lack of controlled animal studies investigating the effects of maternal cigarette smoke exposure on male offspring reproductive health. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6885

6 Samples

Download data: TXT, XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL16446 GPL1261

12 Samples

Download data: CEL, PAIR

(Submitter supplied) Proper DNA-methylation is inevitable for normal development and this epigenetic program is dynamically reprogrammed especially around embryo implantation. Accordingly, embryonic stem cells (ESCs) derived from inner cell mass in pre-implantation embryo exhibit the lowest DNA methylation level compared with differentiated tissue cells to ensure their pluripotency and germ-line potency. Methylated CpG Island Recovery Assay (MIRA) coupled chip DNA methylome analysis demonstrated that Sirt1 deficiency increases the abnormal DNA methylation especially on the a subtype of imprinted and germ-line development genes.

Organism:

Mus musculus

Type:

Methylation profiling by genome tiling array

Platform:

GPL16446

6 Samples

Download data: PAIR, TXT

(Submitter supplied) Stem-cells and transformed cancer cells specifically express a polycomb repressive complex subtype, PRC4 which characteristically contains Sirt1 (Sirtuin-1), a NAD+ dependent class III histone deacetylase (HDAC) and Eed2 isoform as specific members. Analyzing the transcriptiome and methylome analysis of Sirt1 deficient murine ESCs (Sirt1-/- ESC), we demonstrate that these cells repressed specifically on some genomic imprinted and germ-line related genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

6 Samples

Download data: CEL

(Submitter supplied) TFIID is a general transcription factor required for transcription of most protein-coding genes by RNA polymerase II. TAF7L is an X-linked germ cell-specific paralogue of TAF7, which is a generally expressed component of TFIID. Here we report the generation of Taf7l mutant mice by homologous recombination in embryonic stem cells using the Cre-loxP strategy. While spermatogenesis is completed in Taf7l mutant mice, the weight of Taf7l mutant testis is decreased and the amount of sperm in the epididymis is sharply reduced. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2857

Platform:

GPL1261

4 Samples

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Analysis of testes of animals lacking Taf7l. TAF7L is an X-linked germ cell-specific paralog of TAF7, which is a generally expressed component of TFIID. Results provide insight into the role of Taf7l in spermatogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation sets

Platform:

GPL1261

Series:

GSE5510

4 Samples

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