GEO DataSets

(Submitter supplied) Sex in birds is genetically determined, molecular mechanism of which is not well-understood. Their Z sex chromosome (chrZ) lacks whole chromosome inactivation as known for mammalian chrX. To investigate the extent of chrZ dosage compensation and its role in somatic cell’s sex specification, we used a highly-quantitative method and analyzed transcriptional activities of male and female fibroblasts from seven birds. more...

Organism:

Anas platyrhynchos; Meleagris gallopavo; Coturnix japonica; Dromaius novaehollandiae; Pavo cristatus; Taeniopygia guttata; Gallus gallus

Type:

Expression profiling by high throughput sequencing

7 related Platforms

54 Samples

Download data: XLSX

(Submitter supplied) This study focused on a complex transcriptomic comparison of undifferentiated, differentiated and suspension cultured myosatellite cells, aiming to determine the effects of different culture methods on their transcriptome. Modern next-generation sequencing (RNAseq) was used to determine the levels of transcripts in cultures cell samples. Then differential expression and pathway analyses were performed using bionformatical methods. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27940

3 Samples

Download data: XLSX

(Submitter supplied) Salmonella is one of most common causes of bacterial foodborne disease and consumption of contaminated poultry products, including turkey, is one route of exposure. Minimizing colonization of commercial turkeys with Salmonella could reduce the incidence of Salmonella-associated human foodborne illness. Understanding host responses to these bacteria could lead to potential strategies to minimize colonization and thus food safety risk. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28636

7 Samples

Download data: TXT

(Submitter supplied) Consumption of contaminated poultry products is one of the main sources of human campylobacteriosis, of which Campylobacter jejuni subsp. jejuni (C. jejuni) and C. coli are responsible for approximately 98% of the cases. The ceca of commercial turkeys are the main anatomical site where Campylobacter asymptomatically colonizes. We have previously colonized the ceca of commercial turkey poults with C. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29195

32 Samples

Download data: TXT

(Submitter supplied) The male reproductive tract tissue samples were obtained from six 38-wk-old turkeys. The gene expression pattern in turkey testis, epididymis and ductus deferens were determined by high-throughput transcriptome sequencing. The obtained sequence reads were mapped to the turkey genome, and relative expression values were calculated for the analysis of differential expressed genes. Bioinformatics analysis revealed several candidate genes potentially involved in spermatogenesis, spermiogenesis and flagella formation in testis and post-testicular sperm maturation in epididymis and ductus deferens. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27940

18 Samples

Download data: CSV, FA

(Submitter supplied) Gene expression profiling of the premammillary nucleus comparing short day photosensitive, long day photosensitive, and long day photorefractory turkey hens

Organism:

Gallus gallus; Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL19870

12 Samples

Download data: XLS

(Submitter supplied) To validate the use of chicken array for turkey, the ability of species-specific hybridization (SSH, chicken samples-chicken arrays) and cross-species hybridization (CSH, turkey samples-chicken arrays) were compared in the same biological conditions. Reproductively active laying chickens and reproductively inactive non-laying pullets were used to generate the results for SSH. Similarly, reproductively active laying turkeys and reproductively inactive non-laying photorefractory turkeys were used to generate the results for CSH.

Organism:

Gallus gallus; Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL19870

16 Samples

Download data: XLS

(Submitter supplied) The success of turkey breeding for rapid growth rate and larger breast size has coincided with an increasing incidence of a meat quality defect described as pale, soft and exudative (PSE). We hypothesized that this defect, which is associated with an abnormally rapid rate of postmortem metabolism, derives from altered expression of genes involved in metabolic regulation. Our objective was to use deep transcriptome RNA sequence analysis (RNAseq) to identify differentially expressed genes between normal and PSE turkey breasts. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15427

8 Samples

Download data: GTF, TXT

(Submitter supplied) The objective of this study was to determine differential gene expression of turkey breast muscle regarding development of PSE meat defect. Genetically unimproved, random-bred (RBC2) turkeys representing turkeys from 1966, which are smaller and grow slower than modern turkeys, were raised at the Michigan State University (MSU) Poultry farm and harvested at 22 week of age. Breast meat was collected and snap frozen in liquid nitrogen. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

6 Samples

Download data: CSV, GPR

(Submitter supplied) In this study, we directly compared turkey muscle satellite cell gene expression between satellite cells with the gene versican (VCAN) knocked down by siRNA transfection and those transfected with a lipofectamine control using our 6K Turkey Skeletal Muscle Long Oligo (TSKMLO) microarray (GPL9788). The VCAN gene was previously identified as differentially expressed by genetic line and during development of turkey skeletal muscle (Sporer et al., 2011). more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

16 Samples

Download data: CSV, GPR

(Submitter supplied) In this study, we directly compared turkey muscle satellite cell gene expression between satellite cells with the gene Matrix Gla Protein (MGP) knocked down by siRNA transfection and those transfected with a lipofectamine control using our 6K Turkey Skeletal Muscle Long Oligo (TSKMLO) microarray (GPL9788). The MGP gene was previously identified as differentially expressed by genetic line and during development of turkey skeletal muscle (Sporer et al., 2011). more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

16 Samples

Download data: CSV, GPR

(Submitter supplied) In this study, we directly compared turkey muscle satellite cell gene expression between satellite cells with the gene Death-Associated Protein (DAP) knocked down by siRNA transfection and those transfected with a lipofectamine control using our 6K Turkey Skeletal Muscle Long Oligo (TSKMLO) microarray (GPL9788). The DAP gene was previously identified as differentially expressed by genetic line and during development of turkey skeletal muscle (Sporer et al., 2011). more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

16 Samples

Download data: CSV, GPR

(Submitter supplied) Transcriptional profiling of turkey cardiac muscle at 1d post-hatch and 16wk birds of F (growth-selected) and RBC2 (randombred) lines

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

4 Samples

Download data: CSV, GPR

(Submitter supplied) Because of its high plasticity and rapid growth rate, turkey skeletal muscle is an important model for studying mechanisms responsible for vertebrate skeletal muscle development and function. Skeletal muscle is composed of metabolically heterogeneous myofibers that exhibit high plasticity at both the morphological and transcriptional levels. The objective of this study was to employ microarray analysis to elucidate the differential gene expression between the tonic- “red” Anterior latissimus dorsi (ALD) muscle, the phasic- “white” Posterior latissimus dorsi (PLD), and “mixed”-phenotype Biceps femoris (BF) in 1-week and 19-week old male turkeys. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

34 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

70 Samples

Download data: GPR

(Submitter supplied) The purpose of Experiment 2 was two-fold: a) to evaluate a unique set of negative, distance, and mismatch control probes on the newly designed 6K TSKMLO microarray, and b) to identify differences in gene expression between two genetic lines of turkeys that phenotypically display differences in growth. Negative and mismatch controls confirmed specific hybridization using this array, and genes identified as differentially expressed between developmental stages by microarray analysis were confirmed by qRT-PCR. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

30 Samples

Download data: GPR

(Submitter supplied) The purpose of Experiment 1 was two-fold: a) to evaluate a unique set of negative, distance, and mismatch control probes on the newly designed 6K TSKMLO microarray, and b) to identify patterns of gene expression in skeletal muscle during development. Negative and mismatch controls confirmed specific hybridization using this array, and genes identified as differentially expressed between developmental stages by microarray analysis were confirmed by qRT-PCR. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

40 Samples

Download data: GPR

(Submitter supplied) A 6K oligonucleotide microarray (TSKMLO) was designed as a community resource for researchers interested in domestic turkey muscle biology as it pertains to growth and development and their association with downstream meat quality. A series of quality control experiments performed to assess the functionality of the newly constructed array, which included a set of validation hybridizations performed in order to initially assess the functionality and repeatability of the newly designed TSKMLO array. more...

Organism:

Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL9788

4 Samples

Download data

(Submitter supplied) Turkey embryos are very sensitive to perturbations in energy metabolism because they have a wider hatching window than chicken embryos. Mortality of turkey embryos during late-term incubation is high relative to chickens, and many surviving hatchlings have compromised vitality. Intestinal maturation at hatch is also crucial to survival and post-hatch performance. The study of poultry embryo metabolism during the last stages of incubation is difficult due to many shifts and changes that occur in preparation for hatching. more...

Organism:

Gallus gallus; Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL6043

160 Samples

Download data: DAT

(Submitter supplied) Turkey embryos are very sensitive to perturbations in energy metabolism because they have a wider hatching window than chicken embryos. Mortality of turkey embryos during late-term incubation is high relative to chickens, and many surviving hatchlings have compromised vitality. Intestinal maturation at hatch is also crucial to survival and post-hatch performance. The study of poultry embryo metabolism during the last stages of incubation is difficult due to many shifts and changes that occur in preparation for hatching. more...

Organism:

Gallus gallus; Meleagris gallopavo

Type:

Expression profiling by array

Platform:

GPL6043

32 Samples

Download data: DAT