GEO DataSets

(Submitter supplied) Exploring the molecular mechanism underlying plant architecture and breeding new varieties suitable for mechanized harvesting are primary objectives for rapeseed breeders in China. However, few genes controlling plant architecture have been cloned in Brassica napus. In this study, SX3, a scattered-bud B. napus line with a dwarf and compact plant architecture, was characterized. To identify the genes underlying bud arrangement, plant height and branch angle, segregating populations were constructed by crossing SX3 with two clustered-bud lines with a tall and loose plant architecture. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29911

12 Samples

Download data: XLSX

(Submitter supplied) Plants acclimate to drought and water stress through diverse physiological responses, primarily mediated by the hormone abscisic acid (ABA). The closure of stomatal pores on aerial surfaces of plants is one of the rapid responses mediated by ABA to reduce transpirational water loss because plants lose the majority of their water through stomatal pores. Stomatal guard cells have been an important cellular system for studying ABA signaling. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34127

9 Samples

Download data: TXT

(Submitter supplied) Water availability is a major determinant of crop production and rising temperatures from climate change is leading to more extreme droughts. To maximize the impact of irrigation on crop yields, we need to develop varieties that are more tolerant to water limited conditions. We aimed to determine how diverse crop types of the allopolyploid Brassica napus, a species that contains the economically important canola oilseed crop, respond to prolonged water limitation. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29911

355 Samples

Download data: TXT

(Submitter supplied) Oilseed rape (Brassica napus, B. napus) is one of the most important oil crops globally, contributing significantly to the world's supply of vegetable oil. However, its production is severely threatened by Sclerotinia stem rot, a disease caused by the broad-host-range fungus Sclerotinia sclerotiorum (Lib.) de Bary (S. sclerotiorum). We have investigated the gene expression of J9712 and W40-OE2 during different time periods of Sclerotinia sclerotiorum infection through RNA-Seq analysis.

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19731

12 Samples

Download data: TXT

(Submitter supplied) Cultivation of canola at temperatures above the optimum growth temperature of 21°C for prolonged periods, especially during the flowering stage, resulted in several adverse effects, including rapid vegetative growth, reduced viability of female gametophytes, increased seed abortion rate, accelerated embryo development, and a reduction in seed oil composition (Young et al., 2004; Mácová et al., 2022; Secchi et al., 2023). more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29911

28 Samples

Download data: TXT

(Submitter supplied) Programmed cell death (PCD) is a genetically controlled pathway that plants can use to selectively eliminate redundant or damaged cells.The coordinated and sequential actions of lineage-specific transcription factors and epigenetic regulators are essential for the initiation of PCD. We used CRISPR/Cas9-mediated targeted mutagenesis to generate a series of transgene-free mutant lines either with single or double-null bnafah alleles. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

27 Samples

Download data: TXT

(Submitter supplied) Background: MicroRNAs (miRNAs), a class of non-coding small RNAs, are crucial to the regulation of various developmental processes. Plant architecture is a collection of genetically controlled agronomic traits that determine crop production and mechanized harvesting. Although several genes had been found to regulate plant architecture, the mechanisms whereby miRNAs regulate plant architecture in the rapeseed Brassica napus remain unknown. more...

Organism:

Brassica napus

Type:

Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL15579

6 Samples

Download data: TXT, XLSX

(Submitter supplied) Haploid embryos can be induced from cultured immature pollen following stress treatment. In Brassica napus, the application of the histone/lysine deacetylase (HDAC/KDAC) inhibitor trichostatin A (TSA) to pollen cultures enhances the production of differentiated embryos and embryogenic callus when applied together with heat stress (Li et al., 2014). To identify genes associated with the induction of haploid embryogenesis and to investigate which genes may be responsible for TSA-induced lipid and starch accumulation in the embryogenic structures, we compared the transcriptomes of pollen cultures treated with heat stress and 0.05 µM TSA to those of untreated pollen cultures, both at two days.

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19731

6 Samples

Download data: XLSX

(Submitter supplied) Global climate changes on one aspect of extreme temperature records would suddenly reset environmental growth conditions for field-grown crops, which severely affects agronomic and commercial traits. Taking the cold-season preferable crop rapeseed Brassica napus L. for example, low-temperature shocks change endogenous regulatory networks and cause phenotypic damages during most lifespan. Here we screened out two genetic breeding elites with different temperature-dependent germination rates, core germplasms with good germination performance and genetic loci and candidate genes potentially involved in low-temperature tolerant functions for the pre-breeding purpose of cold-tolerant germination. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19731

30 Samples

Download data: TXT

(Submitter supplied) mRNA expression profiling of the embryo, endosperm (micropylar, peripheral, chalazal), and seed coat (outer, inner, chalazal, chalazal proliferating tissue) of the developing Brassica napus seed. Tissues were isolated using laser microdissection (LMD) from Brassica napus seeds at the globular, heart, and mature green stages of seed development.

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

74 Samples

Download data: TXT

(Submitter supplied) It revealed that DEGs associated with floral development, including BnaC06.ARGOS, BnaC09.BPE, BnaA03.AUX1 and BnaAP2, and BnaC05.ERS2 involved in ethylene signalling potentially contribute to the double flower trait in B. napus.

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

6 Samples

Download data: XLSX

(Submitter supplied) Seed quality traits of oilseed rape, Brassica napus (B. napus), exhibit quantitative inheritance determined by its genetic makeup and the environment via the mediation of a complex genetic architecture of hundreds to thousands of genes. Thus, instead of single gene analysis, network-based systems genomics and genetics approaches that combine genotype, phenotype, and molecular phenotypes offer a promising alternative to uncover this complex genetic architecture. more...

Organism:

Brassica napus

Type:

Expression profiling by array

Platform:

GPL15739

200 Samples

Download data: GPR

(Submitter supplied) Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is crucial for profiling histone modifications and transcription factor binding throughout the genome. However, its application in economically important plant organs (EIPOs) such as seeds, fruits and flowers is challenging due to their sturdy cell walls and complex constituents. Here we present advanced ChIP (aChIP), an optimized method that efficiently isolates chromatin from plant tissues while simultaneously removing cell walls and cellular constituents. more...

Organism:

Dianthus caryophyllus; Arabidopsis thaliana; Solanum lycopersicum; Citrus sinensis; Zea mays; Brassica napus; Glycine max; Populus; Solanum tuberosum; Oryza sativa; Saccharum officinarum

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

11 related Platforms

162 Samples

Download data: BROADPEAK, NARROWPEAK

(Submitter supplied) Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a powerful method for profiling histone modifications and transcription factors binding throughout the genome. However, its application in economically important plant organs (EIPOs) such as seeds, fruits, tubers and flowers is challenging due to their sturdy cell walls and complex constituents. Here, we present advanced ChIP (aChIP), an optimized ChIP-seq strategy that efficiently isolates chromatin from plant tissues while simultaneously removing plant cell walls and cellular constituents. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29911

8 Samples

Download data: TXT, XLS

(Submitter supplied) Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a powerful method for profiling histone modifications and transcription factors binding throughout the genome. However, its application in economically important plant organs (EIPOs) such as seeds, fruits, tubers and flowers is challenging due to their sturdy cell walls and complex constituents. Here, we present advanced ChIP (aChIP), an optimized ChIP-seq strategy that efficiently isolates chromatin from plant tissues while simultaneously removing plant cell walls and cellular constituents. more...

Organism:

Glycine max; Dianthus caryophyllus; Solanum lycopersicum; Zea mays; Arabidopsis thaliana; Brassica napus; Solanum tuberosum; Saccharum officinarum; Citrus sinensis; Populus; Oryza sativa

Type:

Genome binding/occupancy profiling by high throughput sequencing

11 related Platforms

154 Samples

Download data: BROADPEAK, NARROWPEAK

(Submitter supplied) Sclerotinia sclerotiorum, the causal agent of white mould, is a necrotrophic fungal pathogen responsible for extensive crop loss. Current control options rely heavily on the application of chemical fungicides that are becoming less effective and may lead to the development of fungal resistance. In the current study, we used a foliar spray application of boron to protect Brassica napus (canola) from S. more...

Organism:

Brassica napus; Sclerotinia sclerotiorum

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL34395 GPL29911

18 Samples

Download data: TXT

(Submitter supplied) Integration of genome wide association studies (GWAS), metabolomics and transcriptomics reveals phenolic acids and flavonoids associated genes and their regulatory elements under drought stress in rapeseed flowers

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34026

12 Samples

Download data: TXT

(Submitter supplied) impaired seedling growth, especially in yellowed seedlings, was not fully rescued in medium supplemented with exogenous sucrose.

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26378

6 Samples

Download data: TXT

(Submitter supplied) This study investigates the transcriptome and physiological responses of rapeseed to post-flowering temperature increases, providing valuable insights into the molecular mechanisms underlying rapeseed tolerance to heat stress. Two rapeseed genotypes, Lumen and Solar, were assessed under control and heat stress conditions in field experiments conducted in Valdivia, Chile. Results showed that seed yield and seed number were negatively affected by heat stress, with genotype-specific responses. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29911

112 Samples

Download data: TXT

(Submitter supplied) UV-B radiation regulates numerous morphogenic, biochemical and physiological responses in plants, and can stimulate some responses typically associated with other abiotic and biotic stimuli, including invertebrate herbivory. Removal of UV-B from the growing environment of various plant species has been found to increase their susceptibility to consumption by invertebrate pests, however, to date, little research has been conducted to investigate the effects of UV-B on crop susceptibility to field pests. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25850

12 Samples

Download data: TXT