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Items: 1 to 20 of 33

  • Did you mean: cotton (2915 items)
1.
Full record GDS5113

Transcription factor Grainyhead-like 2 depletion effect on lung epithelial cell line

Analysis of MLE-15 lung epithelial cells depleted for Grainyhead-like 2. Results provide insight into the role of Grainyhead-like 2 in the morphogenesis and differentiation of lung epithelial cells.
Organism:
Mus musculus
Type:
Expression profiling by array, transformed count, 2 protocol sets
Platform:
GPL6246
Series:
GSE40729
5 Samples
Download data: CEL
2.
Full record GDS3904

Directed differentiation of embryonic stem cells and induced pluripotent stem cells

Analysis of ES cells and iPS cells before (day 0) and after (day 5) endodermal differentiation. E8.25 embryonic definitive endoderm (DE) was also examined. Results provide insight into the relative equivalency of DE derived from ES/iPS cells in vitro and authentic DE from embryos in vivo.
Organism:
Mus musculus
Type:
Expression profiling by array, transformed count, 3 cell type, 3 development stage, 2 strain sets
Platform:
GPL6246
Series:
GSE27087
30 Samples
Download data: CEL
3.

Kinetic microarray of hPSC-derived SCGB3A2+ airway epithelium

(Submitter supplied) We sought to better understand the kinetics of differentiation and the global transcriptomic programs of these labeled cells as they emerge over prolonged culture periods
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL17930
12 Samples
Download data: CEL
Series
Accession:
GSE103517
ID:
200103517
4.

Single cell transcriptomic profiling of pluripotent stem cell-derived SCGB3A2+ airway epithelium reveals fate plasticity

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
78 Samples
Download data
Series
Accession:
GSE103920
ID:
200103920
5.

Single cell transcriptomic profiling of pluripotent stem cell-derived SCGB3A2+ airway epithelium reveals fate plasticity* - Fluidigm C1: bronchospheres day 27*

(Submitter supplied) Many lung diseases involve alterations in the cellular identity of the lung epithelium. Improved insight into airway development and the changes to cellular identity that result from abnormal signaling may therefore improve understanding of the etiology of these complex diseases. We have previously described a protocol to generate epithelial airway spheres from human pluripotent stem cells (hPSCs), but still poorly understand the identity, development, and heterogeneity of these early airway cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
76 Samples
Download data: TXT
Series
Accession:
GSE103919
ID:
200103919
6.

Single cell transcriptomic profiling of pluripotent stem cell-derived SCGB3A2+ airway epithelium reveals fate plasticity *- 10x Chromium: bronchospheres vs. alveolospheres day 41*

(Submitter supplied) Many lung diseases involve alterations in the cellular identity of the lung epithelium. Improved insight into airway development and the changes to cellular identity that result from abnormal signaling may therefore improve understanding of the etiology of these complex diseases. We have previously described a protocol to generate epithelial airway spheres from human pluripotent stem cells (hPSCs), but still poorly understand the identity, development, and heterogeneity of these early airway cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
2 Samples
Download data: MTX, TSV
Series
Accession:
GSE103918
ID:
200103918
7.

Microarray expression data from mouse embryonic stem cells differentiated into Nkx2-1+ lung and thyroid progenitors

(Submitter supplied) The in vitro directed differentiation of pluripotent stem cells (PSCs) through stimulation of developmental signaling pathways can generate mature somatic cell types for basic laboratory studies or regenerative therapies. We used microarrays to detail the global transcriptomes of mouse embryonic stem cells differentiated in vitro into putative thyroid vs lung epithelial lineages using serum-free media supplemented with either BMP4+FGF2 (thyroid media) or BMP4+Wnt3a (lung media.) Differentiated cells carried an Nkx2-1mCherry knock-in reporter to allow sorting of mCherry + vs - cells in each condition on day 14 of differentiation for global transcriptomic profiling.
Organism:
Mus musculus
Type:
Expression profiling by array
Platform:
GPL17791
12 Samples
Download data: CEL
Series
Accession:
GSE92916
ID:
200092916
8.

Generation of mature lung alveolar epithelial cells from human pluripotent stem cells

(Submitter supplied) We perform time-series global transcriptomic profiling of a directed differentiation protocol for generating alveolar epithelial type II cells (AEC2s) from pluripotent stem cells (PSCs). We analyzed 3 different timepoints in the RUES2 differentiation: 1) Day 0 undifferentiated cells, 2) Day 15 lung progenitors highly enriched in NKX2-1+ cells by CD47hi/CD26lo sorting, and 3) the outgrowth of these purified progenitors in 3D culture sorted again on Day 35 based on SFTPCtdTomato+ and SFTPCtdTomato- gating. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16791
21 Samples
Download data: CSV
Series
Accession:
GSE96642
ID:
200096642
9.

Single cell RNA-sequencing of day 15 cells derived from the directed differentiation of human iPSCs towards lung epithelium

(Submitter supplied) It has been postulated that during human fetal development all cells of the lung epithelium derive from an embryonic endodermal NKX2-1+ precursor, however, this hypothesis has not been formally tested due to an inability to purify or track this theorized cell for detailed characterization. Progress has been made in deriving lung epithelial cells from human induced pluripotent stem cells (iPSCs). However, little is known about the heterogeneity or genetic programs of the cells generated using these lung differentiation protocols. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16791
145 Samples
Download data: TXT
Series
Accession:
GSE96106
ID:
200096106
10.

Global gene expression kinetics of early human lung development modeled by directed differentiation of human PSCs using an NKX2-1GFP iPSC reporter

(Submitter supplied) It has been postulated that during human fetal development all cells of the lung epithelium derive from an embryonic endodermal NKX2-1+ precursor, however, this hypothesis has not been formally tested due to an inability to purify or track this theorized cell for detailed characterization. Here we engineer and developmentally differentiate NKX2-1GFP reporter pluripotent stem cells (PSCs) in vitro to generate and isolate a human primordial lung progenitor that expresses NKX2-1 but is initially devoid of markers of differentiated lung lineages. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL17930
27 Samples
Download data: CEL
Series
Accession:
GSE83310
ID:
200083310
11.

Thyroid progenitors are robustly derived from embryonic stem cells through transient, developmental stage-specific overexpression of Nkx2-1

(Submitter supplied) The clinical importance of anterior foregut endoderm (AFE) derivatives, such as thyrocytes, has led to intense research efforts for their derivation through directed differentiation of pluripotent stem cells (PSCs). Here we identify transient overexpression of the transcription factor (TF) NKX2-1 as a powerful inductive signal for the robust derivation of thyrocyte-like cells from mouse PSC-derived AFE. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing
Platform:
GPL17021
8 Samples
Download data: TXT
Series
Accession:
GSE92572
ID:
200092572
12.

Emergence of a developmental stage-dependent human liver disease signature demonstrated by directed differentiation of alpha-1 antitrypsin deficient iPS cells

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens; synthetic construct
Type:
Non-coding RNA profiling by array; Expression profiling by array; Methylation profiling by genome tiling array
Platforms:
GPL14613 GPL13534 GPL6244
81 Samples
Download data: CEL, IDAT
Series
Accession:
GSE66078
ID:
200066078
13.

Emergence of a developmental stage-dependent human liver disease signature demonstrated by directed differentiation of alpha-1 antitrypsin deficient iPS cells [HumanMethylation450]

(Submitter supplied) We monitored 9 pluripotent stem cell lines across three time points of hepatic directed differentiation, representing 3 developmental stages: undifferentiated (T0), definitive endoderm (T5), and early hepatocyte (T24). ESCs (n=3) and patient-derived normal (n=3) or PiZZ (n=3) iPSCs were analyzed in the undifferentiated state (T0), after differentiation to definitive endoderm (T5), and upon reaching hepatic stage (T24) for a total of 27 samples. more...
Organism:
Homo sapiens
Type:
Methylation profiling by genome tiling array
Platform:
GPL13534
27 Samples
Download data: IDAT, TXT
Series
Accession:
GSE66077
ID:
200066077
14.

Emergence of a developmental stage-dependent human liver disease signature demonstrated by directed differentiation of alpha-1 antitrypsin deficient iPS cells [HuGene-1_0-st]

(Submitter supplied) We monitored 9 pluripotent stem cell lines across three time points of hepatic directed differentiation, representing 3 developmental stages: undifferentiated (T0), definitive endoderm (T5), and early hepatocyte (T24). ESCs (n=3) and patient-derived normal (n=3) or PiZZ (n=3) iPSCs were analyzed in the undifferentiated state (T0), after differentiation to definitive endoderm (T5), and upon reaching hepatic stage (T24) for a total of 27 samples. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL6244
27 Samples
Download data: CEL
Series
Accession:
GSE66076
ID:
200066076
15.

Emergence of a developmental stage-dependent human liver disease signature demonstrated by directed differentiation of alpha-1 antitrypsin deficient iPS cells [miRNA-2_0]

(Submitter supplied) We monitored 9 pluripotent stem cell lines across three time points of hepatic directed differentiation, representing 3 developmental stages: undifferentiated (T0), definitive endoderm (T5), and early hepatocyte (T24). ESCs (n=3) and patient-derived normal (n=3) or PiZZ (n=3) iPSCs were analyzed in the undifferentiated state (T0), after differentiation to definitive endoderm (T5), and upon reaching hepatic stage (T24) for a total of 27 samples. more...
Organism:
synthetic construct; Homo sapiens
Type:
Non-coding RNA profiling by array
Platform:
GPL14613
27 Samples
Download data: CEL
Series
Accession:
GSE66075
ID:
200066075
16.

iPS from healthy control and BMPR2 mutant

(Submitter supplied) Comparison between inducible pluripotent stem cells from healthy patients and patients with BMPR2 mutation, at different differentiation stages.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL11532
16 Samples
Download data: CEL, CHP
Series
Accession:
GSE60037
ID:
200060037
17.

Clonal Genetic and Hematopoietic Heterogeneity among Human Induced Pluripotent Stem Cell lines

(Submitter supplied) Induced pluripotent stem cells hold great promise for modeling human hematopoietic diseases. However, intrinsic variability in the capacities of different iPSC lines for hematopoietic development complicates comparative studies and is currently unexplained.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL6244
36 Samples
Download data: CEL, CHP
Series
Accession:
GSE49231
ID:
200049231
18.

Grhl2 regulated genes in the mouse lung epithelial MLE15 cell line

(Submitter supplied) The Grainyhead family of transcription factors controls morphogenesis and differentiation of epithelial cell layers in multicellular organisms by regulating cell junction- and proliferation-related genes. Grainyhead-like 2 (Grhl2) is expressed in developing mouse lung epithelium and is required for normal lung organogenesis. The specific epithelial cells expressing Grhl2 and the genes regulated by Grhl2 in normal lungs are mostly unknown. more...
Organism:
Mus musculus
Type:
Expression profiling by array
Dataset:
GDS5113
Platform:
GPL6246
5 Samples
Download data: CEL
Series
Accession:
GSE40729
ID:
200040729
19.

Efficient derivation of purified lung and thyroid progenitors from embryonic stem cells

(Submitter supplied) Two populations of Nkx2-1+ progenitors in the developing foregut endoderm give rise to the entire post-natal lung and thyroid epithelium, but little is known about these cells, as they are difficult to isolate in a pure form. We demonstrate here the purification and directed differentiation of primordial lung and thyroid progenitors derived from mouse embryonic stem cells (ESCs). Inhibition of TGFβ and BMP signaling, followed by combinatorial stimulation of BMP and FGF signaling can specify these cells efficiently from definitive endodermal precursors. more...
Organism:
Mus musculus
Type:
Expression profiling by array
Platform:
GPL6246
6 Samples
Download data: CEL
Series
Accession:
GSE35063
ID:
200035063
20.

Expression profile of human ESC, ESC-drived definitive endoderm and endodermal progenitor cells

(Submitter supplied) Endodermal progenitor cells (EP cells) are derived from human embryonic stem cell(ESC)-derived definitive endoderm (DE) cells. EP cells are cultured in high BMP media and DE cells are in high Activin media. Both cells can be further differentiated to liver, pancreas, etc. We used microarray to detail the global gene expression profile of DE cells and EP cells to delineate the difference of DE cells and EP cells.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL10739
9 Samples
Download data: CEL, CHP
Series
Accession:
GSE35461
ID:
200035461
  • Did you mean: cotton (2915 items)
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