GSM73539[Accession] - GEO DataSets

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Items: 3

Select item 2000032571.

Time course microarray approach comparing the ectopic expression and loss-of-function of gcm to wildtype

(Submitter supplied) The determination of glial fate in the developing embryonic nervous system of Drosophila is dependent on the master regulatory gene glial cells missing (gcm). gcm encodes a transcription factor and serves as a binary switch by inducing the glial fate and repressing the neuronal pathway. The ectopic expression of gcm throughout the CNS leads to excessive glia on the cost of presumptive neurons, whereas gcm loss-of-function embryos lack nearly all lateral glial cells. more...

Organism:: Drosophila melanogaster

Type:: Expression profiling by array

Platform:: GPL517
58 Samples

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Accession:: GSE3257

ID:: 200003257

Select item 1000005172.

Heidelberg FlyArray

(Submitter supplied) cDNA Microarray from spotted PCR products containing 47,616 features from Drosophila melanogaster and various controls. Primer design was made with GenomePride software. Average length is 500 bp within exonic sequence. Microarray construction The 2nd round PCR-product was spotted in 3x SSC, 150mM NaPO4, 1.5M betaine onto QMT Amino slides (Quantifoil, Jena, Germany) using a MicroGrid II arrayer (BioRobotics, Cambridge, UK) and SMP3 pins (TeleChem International Inc., Sunnyvale, USA). more...