(Submitter supplied) G-quadruplexes (rG4s) are recognized as key structures involved in RNA biology and are linked to neurodegenerative disease and cancer. However, detailed knowledge of rG4s and their binding partners is lacking. Here, a systematic, unbiased affinity proteomics approach identified 80 potential high-confidence interactors of the rG4 structure in the 5’UTR of the NRAS oncogene. Using epitope-tagged protein expression, we validated a subset of interactions including DDX3X, DDX5, DDX17, DHX9, DHX36, FXR1, FXR2, GRSF1 and NSUN5.
more...- Organism:
- Homo sapiens
- Type:
- Expression profiling by high throughput sequencing
- Platform:
- GPL18573
- 18 Samples
Download data: BED, BEDGRAPH, FA, TXT