GSM2354259[Accession] - GEO DataSets

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Items: 4

Select item 2000889151.

Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL19057
27 Samples

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Accession:: GSE88915

ID:: 200088915

PubMed Full text in PMC Similar studies

Select item 2000889132.

Small RNA sequencing of serum-derived RNA for the evaluation of 5' tRNA halves depletion protocols

(Submitter supplied) 5' tRNA halves are abundantly present in small RNA libraries prepared from serum samples, thereby limiting the detection of other small RNA species. In this study, we developed and compared two protocols for the selective depletion of 5' tRNA halves in RNA isolated from murine serum samples. To evaluate the efficacy of both protocols on small RNA sequencing, we performed the two 5' tRNA halves depletion protocols on total RNA isolated from a serum pool collected from healthy mice. more...