GSM2344866[Accession] - GEO DataSets

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Items: 4

Select item 2000889151.

Depletion of tRNA-halves enables effective small RNA sequencing of low-input murine serum samples

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Non-coding RNA profiling by high throughput sequencing

Platform:: GPL19057
27 Samples

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Series

Accession:: GSE88915

ID:: 200088915

PubMed Full text in PMC Similar studies

Select item 2000887282.

Small RNA sequencing of serum collected from mice before and after manifestation of tumor

(Submitter supplied) To investigate the effectiveness of performing 5' tRNA half depletion, we evaluated the association of tumor burden on circulating miRNAs present in mouse serum. Three nude mice (NCr) were orthotopically injected with SH-SY5Y human neuroblastoma cells. Serum samples were collected one week before cell injection and two weeks after injection, when mice had palpable tumors. We performed 5' tRNA half depletion using biotinylated probes and streptavidin beads and prepared small RNA libraries using the TruSeq Small RNA Library Prep kit. more...