(Submitter supplied) The purified DNA probes in spotting buffer were deposited onto aminopropyl silane-coated Nexterion Slide A (Schott, UK) using the Qarray2 arrayer (Genetix, UK) with a 12-pin print head of Genetix aQu 75um split pins (Genetix, UK). Spotted arrays were air-dried for 12+ hours, followed by heating at 80 °C in dry oven for 3 h to immobilise the DNA. Unbound surfaces on the arrays were inactivated by immersing in 60 ml blocking solution: 5× SSC, 0.5% (w/v) bovine serum albumin (BSA, Sigma, UK), 0.5% (w/v) non-fat powdered milk (Tesco, UK) at 50 °C for 45 min, followed by washing 2 times in deionised water.
more...- Organism:
- Homo sapiens; Human gammaherpesvirus 8
- 1 Series
- 15 Samples
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