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Reciprocal gene editing defines targetable mutant H3.3 oncohistone effectors in pediatric glioma

High-grade pediatric gliomas often contain histone H3.3 mutations, but open questions remain about oncogenic mechanisms. To address this gap, we performed ‘reciprocal gene editing’ using CRISPR-Cas9 to introduce H3.3 mutations (K27M, G34R) into...

Species: Homo sapiensType: Genome binding/occupancy profiling by high throughput sequencing

Dataset: GSE129765

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Items: 1 to 20 of 22

1.

Reciprocal gene editing defines targetable mutant H3.3 oncohistone effectors in pediatric glioma

(Submitter supplied) High-grade pediatric gliomas often contain histone H3.3 mutations, but open questions remain about oncogenic mechanisms. To address this gap, we performed ‘reciprocal gene editing’ using CRISPR-Cas9 to introduce H3.3 mutations (K27M, G34R) into H3.3-wildtype brain and glioma cells, while in parallel reverting pre-existing K27M mutations in glioma cells back to wildtype. Analyses of our reciprocally-edited cells indicate that H3.3 mutation leads to specific transcriptomic and epigenetic events, and associated cell biological changes including in xenograft assays. more...
Organism:
Homo sapiens
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL24676
20 Samples
Download data: NARROWPEAK
Series
Accession:
GSE129765
ID:
200129765
2.

Illumina NovaSeq 6000 (Homo sapiens)

Platform
Accession:
GPL24676
ID:
100024676
3.

Line 17 WT

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data
Sample
Accession:
GSM3723013
ID:
303723013
4.

Line 17 Input

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data
Sample
Accession:
GSM3723012
ID:
303723012
5.

Line 13 WT_Input

Organism:
Homo sapiens
Source name:
Line XIII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data
Sample
Accession:
GSM3723011
ID:
303723011
6.

Line 13 Input

Organism:
Homo sapiens
Source name:
Line XIII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data
Sample
Accession:
GSM3723010
ID:
303723010
7.

Line 17 WT H3K27me3 rep2

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721819
ID:
303721819
8.

Line 17 WT H3K27me3 rep1

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721818
ID:
303721818
9.

Line 17 H3K27me3 rep2

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721817
ID:
303721817
10.

Line 17 H3K27me3 rep1

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721816
ID:
303721816
11.

Line 13 WT H3K27me3 rep2

Organism:
Homo sapiens
Source name:
Line XIII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721815
ID:
303721815
12.

Line 13 WT H3K27me3 rep1

Organism:
Homo sapiens
Source name:
Line XIII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721814
ID:
303721814
13.

Line 13 H3K27me3 rep2

Organism:
Homo sapiens
Source name:
Line XIII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721813
ID:
303721813
14.

Line 13 H3K27me3 rep1

Organism:
Homo sapiens
Source name:
Line XIII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721812
ID:
303721812
15.

Line 17 WT H3.3 rep2

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721811
ID:
303721811
16.

Line 17 WT H3.3 rep1

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721810
ID:
303721810
17.

Line 17 H3.3 rep2

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721809
ID:
303721809
18.

Line 17 H3.3 rep1

Organism:
Homo sapiens
Source name:
Line XVII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721808
ID:
303721808
19.

Line 13 WT H3.3 rep2

Organism:
Homo sapiens
Source name:
Line XIII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721807
ID:
303721807
20.

Line 13 WT H3.3 rep1

Organism:
Homo sapiens
Source name:
Line XIII DIPG
Platform:
GPL24676
Series:
GSE129765
Download data: NARROWPEAK
Sample
Accession:
GSM3721806
ID:
303721806
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db=gds|term=GSE129765|query=1|qty=2|blobid=MCID_61a7430642bc9571ab38dc7c|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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