(Submitter supplied) Oligonucleotide printing, microarray slide preparation and processing :Oligonucleotide libraries are printed on poly L Lysine coated microscope slides which are blocked prior to hybridization. Prior to printing, the microscope slides (Gold Seal, VWR, So. Plainfield, NJ) are cleaned with 2.5 M NaOH in 61% ethanol, washed thoroughly, and then coated with 0.01% poly l lysine (Sigma, St. Louis, MO) in filtered 10% phosphate buffered saline without calcium or magnesium (Hyclone, Logan, UT).
more...- Organism:
- Homo sapiens
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