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Items: 2

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1.

In vivo RNA binding sites of the yeast ribosome biogenesis factor Utp23

(Submitter supplied) Purpose: Pre-ribosomal RNA is cleaved at defined sites to release the mature ribosomal RNAs, but the functions of many ribosome biogenesis factors involved in 18S rRNA release are not known. We apply an in vivo cross-linking technique coupled with deep sequencing (CRAC) that captures transcriptome-wide interactions between the yeast PIN domain protein Utp23 and its targets in a living cell. Methods: We apply CRAC to an HTP-tagged Utp23 protein (HTP: His6 - TEV cleavage site - two copies of the z-domain of Protein A) in budding yeast. more...
Organism:
Saccharomyces cerevisiae
Type:
Other
Platforms:
GPL13821 GPL13272
3 Samples
Download data: WIG
2.

Rrp5 binding at multiple sites coordinates pre-rRNA processing and assembly

(Submitter supplied) The CRAC UV crosslinking technique identified numerous pre-rRNA binding sites for the large, highly conserved ribosome synthesis factor Rrp5. Intramolecular complementation has shown that the C-terminal domain (CTD) of Rrp5 is required for pre-rRNA cleavage at sites A0-A2 on the pathway of 18S rRNA synthesis, whereas the N-terminal domain (NTD) is required for A3 cleavage on the pathway of 5.8S/25S rRNA synthesis. more...
Organism:
Saccharomyces cerevisiae BY4741
Type:
Expression profiling by high throughput sequencing
Platform:
GPL17253
4 Samples
Download data: SGR
Series
Accession:
GSE47680
ID:
200047680

Supplemental Content

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