3IE5,1E09,1FM4,2BK0,2I9Y,3E85,1TW0,2FLH,2VNE


Conserved Protein Domain Family
Bet_v1-like
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cd07816: Bet_v1-like 
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Ligand-binding bet_v_1 domain of major pollen allergen of white birch (Betula verrucosa), Bet v 1, and related proteins
This family includes the ligand binding domain of Bet v 1 (the major pollen allergen of white birch, Betula verrucosa) and related proteins. In addition to birch Bet v 1, this family includes other plant intracellular pathogenesis-related class 10 (PR-10) proteins, norcoclaurine synthases (NCSs), cytokinin binding proteins (CSBPs), major latex proteins (MLPs), and ripening-related proteins. It belongs to the SRPBCC (START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC) domain superfamily of proteins that bind hydrophobic ligands. SRPBCC domains have a deep hydrophobic ligand-binding pocket. Members of this family binds a diverse range of ligands. Bet v 1 can bind brassinosteroids, cytokinins, flavonoids and fatty acids. Hyp-1, a PR-10 from Hypericum perforatum/St. John's wort, catalyzes the condensation of two molecules of emodin to the bioactive naphthodianthrone hypericin. NCSs catalyze the condensation of dopamine and 4-hydroxyphenylacetaldehyde to (S)-norcoclaurine, the first committed step in the biosynthesis of benzylisoquinoline alkaloids such as morphine. The role of MLPs is unclear; however, they are associated with fruit and flower development and in pathogen defense responses. A number of PR-10 proteins in this subgroup, including Bet v 1, have in vitro RNase activity, the biological significance of which is unclear. Bet v 1 family proteins have a conserved glycine-rich P (phosphate-binding)-loop proximal to the entrance of the ligand-binding pocket. However, its conformation differs from that of the canonical P-loop structure found in nucleotide-binding proteins. Several PR-10 members including Bet v1 are allergenic. Cross-reactivity of Bet v 1 with homologs from plant foods results in birch-fruit syndrome.
Links
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Statistics
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PSSM-Id: 176858
Aligned: 98 rows
Threshold Bit Score: 93.7932
Created: 20-Jun-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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hydrophobicglycine-rich
Conserved site includes 35 residues -Click on image for an interactive view with Cn3D
Feature 1:hydrophobic ligand binding site
Evidence:
  • Comment:This family binds a diverse range of ligands.
  • Structure:2FLH_A; Vigna radiate cytokinin-specific binding protein (CSBP) binds two trans-zeatin molecules, contacts at 4A.
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  • Structure:3E85_A; Lupinus luteus PR-10 protein (LlPR-10.2B) binds a synthetic cytokinin (four N,N'-diphenylurea molecules), contacts at 4A.
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  • Structure:1FM4_A; birch pollen allergen Bet V 1-L binds two deoxycholic acid molecules, contacts at 4A.
    - View structure with Cn3D
  • Comment:Bet v 1-L is believed to be involved in the transport of plant steroids. Although deoxycholate is not the natural ligand for Bet v 1, it is structurally very similar to the plant steroids, brassinosteroids.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1          #   #             ##  #     #       #                 # ##     #  # #         
3IE5_A        10 YTIVKEEESPI-APHRLFKALVLERh--QVLVKAqphvFKSGEIIEGDGGVGTVTKITFVDGh-pLTYMLHKFDEIDaan 85  Hypericum perfo...
2FLH_A         3 KEFNTQTELSV-RLEALWAVLSKDFi--TVVPKVlphiVKDVQLIEGDGGVGTILIFNFLPEv-sPSYQREEITEFDess 78  Vigna radiata
2VNE_A        29 KVIHHELEVAA-SADDIWTVYSWPGl-aKHLPDLlp-gAFEKLEIIGDGGVGTILDXTFVPGe-fPHEYKEKFILVDneh 104 Thalictrum flavum
CAH67978       3 GSVCHELETGL-PAAEVWEVYGGFLv-aQLLPQLvpevFSKVELVEGDGGVGSVLHVVFAPGahrGEFMKEKFIKIDnen 80  Indian rice
XP_002330429   3 KEVKTQVNVGV-EVDVLWKAFGKDLk--DILPKMmpnlVKDADMLEGDGGLDTVYLFNFGPGlktMTYQKERVTEFDefv 79  black cottonwood
AAX56076       3 GHVTNEMDVVGaSADEIWAVYGSKDlpkLIITLApg-vFEKIDYIEGDGGQGTILHIVMAQGipePREWREKFVTVDhcr 81  opium poppy
AAX56304      41 KEIRYDLEVPT-SADSIWSVYSCPDiprLLRDVLlpgvFQKLDVIEGNGGVGTVLDIVFPPGa-vPRSYKEKFVNINhek 118 opium poppy
AAX56075       3 YELINEFDVGA-SADDVWAVYSSPDl-pRLIVELlpgvFKKIDVVEGNGGLGTVLHLVYPHGs-vPLSYKEKFVTMDnhk 79  opium poppy
CAH67977       3 GSQSHELETDV-PASELWKIYGTLRa-aELLPELlphiLAKVELVTGDGGVGTIVRLTFPPGipgLQSYKEKFIKVDnkn 80  Indian rice
XP_002447025  10 GSICHEFETGL-PAADVWGVYGTLLf-gKLMPQLlpevVSKVELLEGDGGAGSVLLITFPPGtpgSETFKEKFIKVDnen 87  sorghum

Feature 1          # # #   ##       # # # #         # # # #                   ## ##  ##  ###     
3IE5_A        86 FYCKYTLFEGDVlrdnIEKVVYEVKLEAv-GGGSKGKITVTYHPKPgc--------tvNEEEVKIGEKKA-YEFYKQVEE 155 Hypericum perfo...
2FLH_A        79 HEIGLQVIEGGYlsqgLSYYKTTFKLSEieEDKTLVNVKISYDHDSdi---------eEKVTPTKTSQST-LMYLRRLER 148 Vigna radiata
2VNE_A       105 RLKKVQXIEGGYldlgVTYYXDTIHVVPtgKDSCVIKSSTEYHVKPef---------vKIVEPLITTGPL-AAXADAISK 174 Thalictrum flavum
CAH67978      81 YIKEAEVIEGGFldqgFKKYVVRIEIIGktENSSVLRSTIKFEAEDas---------kASSVSTGGLAAT-AEAVTKYMR 150 Indian rice
XP_002330429  80 HRIGLEVIEGGHlnhgFSHYKTTFQLTStgEQETLIDVTISYESQV------------EEDTVPSNSASStLVFIKHMEN 147 black cottonwood
AAX56076      82 RVKQLQQIQGGYldmgFSLYKVTYEILPkdKNSCIIKSTVQIDLDDk----------nFEANARLITVDAmWGMAKSIVQ 151 opium poppy
AAX56304     119 RLKEVIMIEGGYldmgCTFYMDRIHIFEktPNSCVIESSIIYEVKEeyagkmaklittEPLESMAEVISG-YVLKKRLQV 197 opium poppy
AAX56075      80 RLKEVRQIEGGYlemgCTFYMDSFQILKktHDSCTIRSITKYEVSAel---------aEKVSSHISVESL-VDMARAISK 149 opium poppy
CAH67977      81 YVKEAEAVEGDIlklgFLSYMIRFEIIRkgANTSVIRSTIEYEIGDe----------hPELQAMVSTASL-AATAEKFAE 149 Indian rice
XP_002447025  88 YIKETIVTEGGAldhgFQKYMVRIEIMGkeDKTSIVRSTMEYEVDDeha-------dtPPVFSTDGLATI-AKAITKYIK 159 sorghum

Feature 1          
3IE5_A       156 YL 157 Hypericum perforatum
2FLH_A       149 YL 150 Vigna radiata
2VNE_A       175 LV 176 Thalictrum flavum
CAH67978     151 EQ 152 Indian rice
XP_002330429 148 YL 149 black cottonwood
AAX56076     152 YV 153 opium poppy
AAX56304     198 FG 199 opium poppy
AAX56075     150 YV 151 opium poppy
CAH67977     150 YI 151 Indian rice
XP_002447025 160 EQ 161 sorghum

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