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Select item 14589841.

GeneReviews® [Internet].

Adam MP, Bick S, Mirzaa GM, et al., editors.

Seattle (WA): University of Washington, Seattle; 1993-2026.

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Select item 44036682.

StatPearls [Internet].

Treasure Island (FL): StatPearls Publishing; 2025 Jan-.

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Select item 14152803.

Madame Curie Bioscience Database [Internet].

Austin (TX): Landes Bioscience; 2000-2013.

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Select item 26591824.

Database of Abstracts of Reviews of Effects (DARE): Quality-assessed Reviews [Internet].

York (UK): Centre for Reviews and Dissemination (UK); 1995-.

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Select item 16686905.

Holland-Frei Cancer Medicine. 6th edition.

Kufe DW, Pollock RE, Weichselbaum RR, et al., editors.

Hamilton (ON): BC Decker; 2003.

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Select item 54056586.

Immunogenetics: Methods and Protocols [Internet].

Langerak AW, editor.

New York: Humana; 2022.

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Select item 23639747.

Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis.

Arvin A, Campadelli-Fiume G, Mocarski E, et al., editors.

Cambridge: Cambridge University Press; 2007.

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Select item 40947248.

BioWatch PCR Assays: Building Confidence, Ensuring Reliability: Abbreviated Version.

Committee on PCR Standards for the BioWatch Program; Board on Life Sciences; Division on Earth and Life Studies; Board on Health Sciences Policy; Institute of Medicine; National Research Council.

Washington (DC): National Academies Press (US); 2015 Jun 26.

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Select item 18602429.

WormBook: The Online Review of C. elegans Biology [Internet].

Pasadena (CA): WormBook; 2005-2018.

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Select item 431748810.

DNA Mismatch Repair Deficiency Tumour Testing for Patients With Colorectal Cancer: A Health Technology Assessment [Internet].

Assasi N, Blackhouse G, Campbell K, et al.

Ottawa (ON): Canadian Agency for Drugs and Technologies in Health; 2016 Aug. (CADTH Optimal Use Report, No. 5.3b.)

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Select item 406499611.

Rapid detection of health-care-associated bloodstream infection in critical care using multipathogen real-time polymerase chain reaction technology: a diagnostic accuracy study and systematic review.

Warhurst G, Dunn G, Chadwick P, et al.

Southampton (UK): NIHR Journals Library; 2015 May. (Health Technology Assessment, No. 19.35.)

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Select item 412963812.

Human Papillomaviruses.

IARC Working Group on the Evaluation of Carcinogenic Risks to Humans.

Lyon (FR): International Agency for Research on Cancer; 2007. (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 90.)

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Select item 333779613.

Can rapid integrated polymerase chain reaction-based diagnostics for gastrointestinal pathogens improve routine hospital infection control practice? A diagnostic study.

Pankhurst L, Macfarlane-Smith L, Buchanan J, et al.

Southampton (UK): NIHR Journals Library; 2014 Aug. (Health Technology Assessment, No. 18.53.)

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Select item 235805214.

Probe Reports from the NIH Molecular Libraries Program [Internet].

Bethesda (MD): National Center for Biotechnology Information (US); 2010-.

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Select item 568529615.

The EBMT Handbook: Hematopoietic Cell Transplantation and Cellular Therapies [Internet]. 8th edition.

Sureda A, Corbacioglu S, Greco R, et al., editors.

Cham (CH): Springer; 2024.

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Select item 332687216.

Randomised controlled trial and health economic evaluation of the impact of diagnostic testing for influenza, respiratory syncytial virus and Streptococcus pneumoniae infection on the management of acute admissions in the elderly and high-risk 18- to 64-year-olds.

Nicholson KG, Abrams KR, Batham S, et al.

Southampton (UK): NIHR Journals Library; 2014 May. (Health Technology Assessment, No. 18.36.)

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Select item 333135417.

Epidermal growth factor receptor tyrosine kinase (EGFR-TK) mutation testing in adults with locally advanced or metastatic non-small cell lung cancer: a systematic review and cost-effectiveness analysis.

Westwood M, Joore M, Whiting P, et al.

Southampton (UK): NIHR Journals Library; 2014 May. (Health Technology Assessment, No. 18.32.)

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Select item 340129418.

Endotext [Internet].

Feingold KR, Adler RA, Ahmed SF, et al., editors.

South Dartmouth (MA): MDText.com, Inc.; 2000-.

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Select item 438190019.

Human Papillomaviruses.

IARC Working Group on the Evaluation of Carcinogenic Risks to Humans.

Lyon (FR): International Agency for Research on Cancer; 1995. (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 64.)

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Select item 420867020.

Guidelines for Stopping Mass Drug Administration and Verifying Elimination of Human Onchocerciasis: Criteria and Procedures.

Geneva: World Health Organization; 2016.

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Supplemental Content

Images search in Bookshelf

FIGURE 4-1PCR Assay. Several key steps precede the PCR amplification reaction. When combined with a detection mode, these steps form a complete PCR assay

The Framework that Surrounds the PCR Reaction Affects Performance.

Committee on PCR Standards for the BioWatch Program; Board on Life Sciences; Division on Earth and Life Studies; Board on Health Sciences Policy; Institute of Medicine; National Research Council. BioWatch PCR Assays: Building Confidence, Ensuring Reliability: Abbreviated Version. Washington (DC): National Academies Press (US); 2015 Jun 26.
Figure 4.30Analysing the results of a PCR by agarose gel electrophoresis

The PCR has been carried out in a microfuge tube. A sample is loaded into lane 2 of an agarose gel. Lane 1 contains DNA size markers, and lane 3 contains a sample of a PCR done by a colleague. After electrophoresis, the gel is stained with ethidium bromide (see Technical Note 2.1). Lane 2 contains a single band of the expected size, showing that the PCR has been successful. In lane 3 there is no band - this PCR has not worked.

Studying DNA.

Brown TA. Genomes. 2nd edition. Oxford: Wiley-Liss; 2002.
Figure 6.4One way of using PCR to prepare template DNA for chain termination sequencing

The PCR is carried out with one normal primer (shown in red), and one primer that is labeled with a metallic bead (shown in brown). After PCR, the labeled strands are purified with a magnetic device. For more details about PCR, see Section 4.3.

Sequencing Genomes.

Brown TA. Genomes. 2nd edition. Oxford: Wiley-Liss; 2002.
BOX FIGURESeveral key steps precede the PCR amplification

When combined with a detection mode, the set of steps forms a complete PCR assay. The report focuses primarily on PCR amplification and subsequent steps

Summary.

Committee on PCR Standards for the BioWatch Program; Board on Life Sciences; Division on Earth and Life Studies; Board on Health Sciences Policy; Institute of Medicine; National Research Council. BioWatch PCR Assays: Building Confidence, Ensuring Reliability: Abbreviated Version. Washington (DC): National Academies Press (US); 2015 Jun 26.