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Select item 23580521.

Probe Reports from the NIH Molecular Libraries Program [Internet].

Bethesda (MD): National Center for Biotechnology Information (US); 2010-.

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Select item 46267142.

Calcium Entry Channels in Non-Excitable Cells.

Kozak JA, Putney JW Jr, editors.

Boca Raton (FL): CRC Press/Taylor & Francis; 2018.

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Select item 56322163.

National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet].

Bethesda (MD): National Cancer Institute (US); 2005 May 1-.

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Select item 14152804.

Madame Curie Bioscience Database [Internet].

Austin (TX): Landes Bioscience; 2000-2013.

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Select item 49579705.

Amino acids: Neonatal parenteral nutrition: Evidence review D2.

National Guideline Alliance (UK).

London: National Institute for Health and Care Excellence (NICE); 2020 Feb. (NICE Guideline, No. 154.)

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Select item 51860766.

New technologies for diagnosing active TB: the VANTDET diagnostic accuracy study.

Halliday A, Jain P, Hoang L, et al.

Southampton (UK): NIHR Journals Library; 2021 Apr. (Efficacy and Mechanism Evaluation, No. 8.5.)

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Select item 49582547.

Lipids: Neonatal parenteral nutrition: Evidence review D3.

National Guideline Alliance (UK).

London: National Institute for Health and Care Excellence (NICE); 2020 Feb. (NICE Guideline, No. 154.)

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Select item 49581848.

Ratio of carbohydrates to lipids: Neonatal parenteral nutrition: Evidence review D8.

National Guideline Alliance (UK).

London: National Institute for Health and Care Excellence (NICE); 2020 Feb. (NICE Guideline, No. 154.)

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Select item 49581189.

Ratio of non-nitrogen energy to nitrogen: Neonatal parenteral nutrition: Evidence review D7.

National Guideline Alliance (UK).

London: National Institute for Health and Care Excellence (NICE); 2020 Feb. (NICE Guideline, No. 154.)

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Select item 495801410.

Glucose: Neonatal parenteral nutrition: Evidence review D1.

National Guideline Alliance (UK).

London: National Institute for Health and Care Excellence (NICE); 2020 Feb. (NICE Guideline, No. 154.)

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Select item 435088811.

A randomised placebo-controlled trial investigating efficacy and mechanisms of low-dose intradermal allergen immunotherapy in treatment of seasonal allergic rhinitis.

Slovick A, Douiri A, Muir R, et al.

Southampton (UK): NIHR Journals Library; 2016 Dec. (Efficacy and Mechanism Evaluation, No. 3.10.)

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Select item 464581012.

Signaling Mechanisms Regulating T Cell Diversity and Function.

Soboloff J, Kappes DJ, editors.

Boca Raton (FL): CRC Press/Taylor & Francis; 2018.

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Select item 568529613.

The EBMT Handbook: Hematopoietic Cell Transplantation and Cellular Therapies [Internet]. 8th edition.

Sureda A, Corbacioglu S, Greco R, et al., editors.

Cham (CH): Springer; 2024.

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Select item 454370414.

The Impact of Food Bioactives on Health: in vitro and ex vivo models [Internet].

Verhoeckx K, Cotter P, López-Expósito I, et al., editors.

Cham (CH): Springer; 2015.

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Select item 290697615.

TRP Channels.

Zhu MX, editor.

Boca Raton (FL): CRC Press/Taylor & Francis; 2011.

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Select item 313182016.

Regenerative Medicine.

National Academy of Sciences (US).

Washington (DC): National Academies Press (US); 2003.

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Select item 236397417.

Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis.

Arvin A, Campadelli-Fiume G, Mocarski E, et al., editors.

Cambridge: Cambridge University Press; 2007.

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Select item 166869018.

Holland-Frei Cancer Medicine. 6th edition.

Kufe DW, Pollock RE, Weichselbaum RR, et al., editors.

Hamilton (ON): BC Decker; 2003.

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Select item 497298219.

Assessment of Long-Term Health Effects of Antimalarial Drugs When Used for Prophylaxis.

National Academies of Sciences, Engineering, and Medicine; Health and Medicine Division; Committee to Review Long-Term Health Effects of Antimalarial Drugs; Board on Population Health and Public Health Practice; Styka AN, Savitz DA, editors.

Washington (DC): National Academies Press (US); 2020 Feb 25.

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Select item 571504620.

Aspartame, methyleugenol, and isoeugenol.

IARC Working Group on the Identification of Carcinogenic Hazards to Humans.

Lyon (FR): International Agency for Research on Cancer; 2024. (IARC Monographs on the Identification of Carcinogenic Hazards to Humans, No. 134.)

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b

YFP-STIM1-CFP construct: This probe was used to determine the fluorescence intensity of equimolar CFP and YFP while assuring minimal intramolecular CFP-YFP FRET since under conditions of STIM1 activation (emptying of stores with ionomycin), the two fluorophores are maximally separated (>15 nm) at each end of the STIM1 molecule.

Assessing the Molecular Nature of the STIM1/Orai1 Coupling Interface Using FRET Approaches.

Kozak JA, Putney JW Jr, editors. Calcium Entry Channels in Non-Excitable Cells. Boca Raton (FL): CRC Press/Taylor & Francis; 2018.
Figure 4Modulation of endogenous IL 17 and IL-23R by new RORγ inverse agonist probe (CID 51035449; ML310/SR2211) in EL-4 cells

Expression of endogenous IL 17A and IL-23R in EL-4 cells was reduced by treatment with ML310. EL-4 cells were pretreated with Digoxin (5 μM) or Probe ML310 (5 μM) or DMSO for 20 hours followed by stimulation with PMA and ionomycin for 5 hours. IL-17A (a) and IL-23R (b) mRNA expression was quantitated and normalized to GAPDH, or intracellular IL-17 protein (c) expression was measured. The results are shown as mean ± SEM; *p < 0.05, ***p < 0.0005.

Identification of a novel selective inverse agonist probe and analogs for the Retinoic acid receptor-related Orphan Receptor Gamma (RORγ).

Probe Reports from the NIH Molecular Libraries Program [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2010-.
Figure 12.4

Meiosis is associated with inhibition of STIM1 clustering and Orai1 internalization. Oocyte were injected with mCherry-STIM1 and GFP-Orai1 (a) and matured into eggs (meiosis) (b). Images show the distribution of Orai1 and STIM1 before and after store depletion with TPEN (5 mM). Images show orthogonal section across a stack of confocal images representing a cross section across the plasma membrane and cortical cytoplasm. Whereas STIM1-Orai1 cocluster in oocytes (a), this clustering is inhibited in eggs (b). The ER remodels in eggs and forms large membrane patches to which STIM1 localizes. Orai1 is enriched in intracellular vesicles in eggs. Scale bar is 2 μm. (c) SOCE inactivates during meiosis. Control oocytes or oocytes and eggs injected with STIM1 and Orai1 were treated with ionomycin (10 μM) to deplete intracellular Ca²⁺ stores and activate SOCE. SOCE activates in oocytes but not in eggs overexpressing STIM1 and Orai1.

Regulation and Role of Store-Operated Ca2+ Entry in Cellular Proliferation.

Kozak JA, Putney JW Jr, editors. Calcium Entry Channels in Non-Excitable Cells. Boca Raton (FL): CRC Press/Taylor & Francis; 2018.
Figure 20.1Specificity and selectivity of Fura Red AMTM to Ca2+ under conditions of classical in vivo cell imaging experiments

Macrophages were incubated with the dye, which was taken up into the cytoplasm. Then the release of Ca²⁺ and eventually other cations from intracellular compartments (such as the endoplasmic reticulum) was stimulated by adding phorbol myristate acetate and/or ionomycin. Thereafter, cells were washed and lysed, and the lysate was injected into a size exclusion chromatography (SEC) column. SEC was run under conditions that preserved the metal-bound state. High molecular weight compounds elute first; low molecular weight compounds (e.g., free Ca²⁺) last. Shown are SEC chromatograms with fluorescence detection (FL) and ICP-MS detection of iron, copper, zinc, and calcium. The Ca peak at 26 min must belong to free Ca²⁺ and cannot be attributable to Fura Red Ca complexes, as no fluorescence is detected. None of the fluorescence signals can be attributed to Ca²⁺ because calcium was not simultaneously detected. Therefore, Fura Red AM^TM may detect other ions such as Zn²⁺, but not Ca²⁺. Modified after Landero-Figueroa et al. (2013), with permission of the Royal Society of Chemistry.

Methods and Technologies for Studying Metals in Biological Systems.

Nriagu JO, Skaar EP, editors. Trace Metals and Infectious Diseases [Internet]. Cambridge (MA): MIT Press; 2015.