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Select item 14152801.

Madame Curie Bioscience Database [Internet].

Austin (TX): Landes Bioscience; 2000-2013.

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Select item 31310662.

Safety of Genetically Engineered Foods: Approaches to Assessing Unintended Health Effects.

National Research Council (US) Committee on Identifying and Assessing Unintended Effects of Genetically Engineered Foods on Human Health.

Washington (DC): National Academies Press (US); 2004.

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Select item 23639743.

Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis.

Arvin A, Campadelli-Fiume G, Mocarski E, et al., editors.

Cambridge: Cambridge University Press; 2007.

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Select item 16686904.

Holland-Frei Cancer Medicine. 6th edition.

Kufe DW, Pollock RE, Weichselbaum RR, et al., editors.

Hamilton (ON): BC Decker; 2003.

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Select item 18957885.

Molecular Biology of the Cell. 4th edition.

Alberts B, Johnson A, Lewis J, et al.

New York: Garland Science; 2002.

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Select item 30666406.

Oversight and Review of Clinical Gene Transfer Protocols: Assessing the Role of the Recombinant DNA Advisory Committee.

Committee on the Independent Review and Assessment of the Activities of the NIH Recombinant DNA Advisory Committee; Board on Health Sciences Policy; Institute of Medicine; Lenzi RN, Altevogt BM, Gostin LO, editors.

Washington (DC): National Academies Press (US); 2014 Mar 27.

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Select item 34012947.

Endotext [Internet].

Feingold KR, Ahmed SF, Anawalt B, et al., editors.

South Dartmouth (MA): MDText.com, Inc.; 2000-.

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Select item 14589848.

GeneReviews® [Internet].

Adam MP, Feldman J, Mirzaa GM, et al., editors.

Seattle (WA): University of Washington, Seattle; 1993-2025.

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Select item 16868409.

Retroviruses.

Coffin JM, Hughes SH, Varmus HE, editors.

Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997.

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Select item 159952410.

The Cell: A Molecular Approach. 2nd edition.

Cooper GM.

Sunderland (MA): Sinauer Associates; 2000.

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Select item 328097011.

Biotechnology and the Food Supply: Proceedings of a Symposium.

National Research Council (US) Commission on Life Sciences.

Washington (DC): National Academies Press (US); 1988.

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Select item 186024212.

WormBook: The Online Review of C. elegans Biology [Internet].

Pasadena (CA): WormBook; 2005-2018.

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Select item 440366813.

StatPearls [Internet].

Treasure Island (FL): StatPearls Publishing; 2025 Jan-.

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Select item 315091214.

Opportunities in Biology.

National Research Council (US) Committee on Research Opportunities in Biology.

Washington (DC): National Academies Press (US); 1989.

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Select item 144161815.

Helicobacter pylori: Physiology and Genetics.

Mobley HLT, Mendz GL, Hazell SL, editors.

Washington (DC): ASM Press; 2001.

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Select item 317101416.

New Vaccine Development Establishing Priorities: Volume II: Diseases of Importance in Developing Countries.

Institute of Medicine (US) Committee on Issues and Priorities for New Vaccine Development.

Washington (DC): National Academies Press (US); 1986.

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Select item 485381617.

LiverTox: Clinical and Research Information on Drug-Induced Liver Injury [Internet].

Bethesda (MD): National Institute of Diabetes and Digestive and Kidney Diseases; 2012-.

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Select item 158168618.

Medical Microbiology. 4th edition.

Baron S, editor.

Galveston (TX): University of Texas Medical Branch at Galveston; 1996.

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Select item 140036219.

Hepatitis C Viruses: Genomes and Molecular Biology.

Tan SL, editor.

Norfolk (UK): Horizon Bioscience; 2006.

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Select item 191116420.

Genomes. 2nd edition.

Brown TA.

Oxford: Wiley-Liss; 2002.

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Figure 3.18Generation of a recombinant DNA molecule

A fragment of human DNA is inserted into a λ DNA vector. The resulting recombinant molecule is then introduced into E. coli, where it replicates to yield recombinant progeny phage containing the human DNA insert.

Recombinant DNA.

Cooper GM. The Cell: A Molecular Approach. 2nd edition. Sunderland (MA): Sinauer Associates; 2000.
Figure 7-21The formation of a recombinant DNA molecule

The cohesive ends produced by many kinds of restriction nucleases allow two DNA fragments to join by complementary base-pairing (see Figure 7-2). DNA fragments joined in this way can be covalently linked in a highly efficient reaction catalyzed by the enzyme DNA ligase. In this example a recombinant plasmid DNA molecule containing a chromosomal DNA insert is formed.

DNA Cloning .

Alberts B, Bray D, Lewis J, et al. Molecular Biology of the Cell. 3rd edition. New York: Garland Science; 1994.
Figure 3.19Joining of DNA molecules

Vector and insert DNAs are digested with a restriction endonuclease (such as EcoRI), which cleaves at staggered sites leaving overhanging single-stranded tails. Vector and insert DNAs can then associate by complementary base pairing, and covalent joining of the DNA strands by DNA ligase yields a recombinant molecule.

Recombinant DNA.

Cooper GM. The Cell: A Molecular Approach. 2nd edition. Sunderland (MA): Sinauer Associates; 2000.
Figure 3.21Cloning in bacteriophage λ vectors

The vector contains a restriction site (e.g., an EcoRI site) for insertion of cloned DNA. In addition, cos sites (cohesive ends), which are required for packaging DNA into phage particles, are present on both ends of the vector DNA. Insert DNA (e.g., human DNA) is ligated to the vector, and the recombinant molecules are packaged into phage particles by being mixed with phage proteins. The recombinant phages are then used to infect E. coli. Each recombinant phage, which carries a unique insert of cloned DNA, forms a single plaque in the infected bacterial culture. Progeny phage carrying unique DNA inserts can then be isolated from individual plaques and grown in large quantity.

Recombinant DNA.

Cooper GM. The Cell: A Molecular Approach. 2nd edition. Sunderland (MA): Sinauer Associates; 2000.