"DNA Footprinting" - Books

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See met5 (DNA) DNA methyl transferase5 in the Gene database

Results: 1 to 20 of 34 books (93 items)

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Select item 14152801.

Madame Curie Bioscience Database [Internet].

Austin (TX): Landes Bioscience; 2000-2013.

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Select item 19111642.

Genomes. 2nd edition.

Brown TA.

Oxford: Wiley-Liss; 2002.

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Select item 15543983.

Mapping Protein/DNA Interactions by Cross-Linking [Internet].

Paris: Institut national de la santé et de la recherche médicale; 2001.

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Select item 18957884.

Molecular Biology of the Cell. 4th edition.

Alberts B, Johnson A, Lewis J, et al.

New York: Garland Science; 2002.

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Select item 15995245.

The Cell: A Molecular Approach. 2nd edition.

Cooper GM.

Sunderland (MA): Sinauer Associates; 2000.

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Select item 54052286.

Whole-Body Regeneration: Methods and Protocols [Internet].

Blanchoud S, Galliot B, editors.

New York (NY): Humana; 2022.

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Select item 16868407.

Retroviruses.

Coffin JM, Hughes SH, Varmus HE, editors.

Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997.

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Select item 23639748.

Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis.

Arvin A, Campadelli-Fiume G, Mocarski E, et al., editors.

Cambridge: Cambridge University Press; 2007.

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Select item 31898349.

Self-Perpetuating Structural States in Biology, Disease, and Genetics.

National Academy of Sciences (US); Lindquist S, Henikoff S, editors.

Washington (DC): National Academies Press (US); 2002.

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Select item 312554610.

New Frontiers in Contraceptive Research: A Blueprint for Action.

Institute of Medicine (US) Committee on New Frontiers in Contraceptive Research; Nass SJ, Strauss JF III, editors.

Washington (DC): National Academies Press (US); 2004.

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Select item 297840811.

Antimicrobial Resistance: Issues and Options: Workshop Report.

Institute of Medicine (US) Forum on Emerging Infections; Harrison PF, Lederberg J, editors.

Washington (DC): National Academies Press (US); 1998.

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Select item 474751812.

Assessing Rare Variation in Complex Traits: Design and Analysis of Genetic Studies [Internet].

Zeggini E, Morris A, editors.

New York (NY): Springer; 2015.

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Select item 424012013.

Veterans and Agent Orange: Update 2014.

Committee to Review the Health Effects in Vietnam Veterans of Exposure to Herbicides (Tenth Biennial Update); Board on the Health of Select Populations; Institute of Medicine; National Academies of Sciences, Engineering, and Medicine.

Washington (DC): National Academies Press (US); 2016 Mar 29.

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Select item 437016414.

Polychlorinated Dibenzo-para-dioxins and Polychlorinated Dibenzofurans.

IARC Working Group on the Evaluation of Carcinogenic Risks to Humans.

Lyon (FR): International Agency for Research on Cancer; 1997. (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 69.)

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Select item 420372015.

Streptococcus pyogenes : Basic Biology to Clinical Manifestations [Internet].

Ferretti JJ, Stevens DL, Fischetti VA, editors.

Oklahoma City (OK): University of Oklahoma Health Sciences Center; 2016-.

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Select item 443423616.

The Chemistry of Microbiomes: Proceedings of a Seminar Series.

National Academies of Sciences, Engineering, and Medicine; Division on Earth and Life Studies; Board on Chemical Sciences and Technology; Chemical Sciences Roundtable.

Washington (DC): National Academies Press (US); 2017 Jul 19.

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Select item 446506417.

Autoimmunity: From Bench to Bedside [Internet].

Anaya JM, Shoenfeld Y, Rojas-Villarraga A, et al., editors.

Bogota (Colombia): El Rosario University Press; 2013 Jul 18.

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Select item 317431618.

Veterans and Agent Orange: Update 2008.

Institute of Medicine (US) Committee to Review the Health Effects in Vietnam Veterans of Exposure to Herbicides (Seventh Biennial Update).

Washington (DC): National Academies Press (US); 2009.

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Select item 150165019.

NCBI News [Internet].

Bethesda (MD): National Center for Biotechnology Information (US); 1991-2012.

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Select item 541465420.

Physics of Life.

National Academies of Sciences, Engineering, and Medicine; Division on Earth and Life Studies; Division on Engineering and Physical Sciences; Board on Life Sciences; Board on Physics and Astronomy; Committee on Biological Physics/Physics of Living Systems: A Decadal Survey.

Washington (DC): National Academies Press (US); 2022 Mar 23.

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Supplemental Content

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Figure 6.3DNA footprinting

A sample containing fragments of DNA radiolabeled at one end is divided into two, and one half of the sample is incubated with a protein that binds to a specific DNA sequence within the fragment. Both samples are then digested with DNase, under conditions such that the DNase introduces an average of one cut per molecule. The region of DNA bound to the protein is protected from DNase digestion. The DNA-protein complexes are then denatured, and the sizes of the radiolabeled DNA fragments produced by DNase digestion are analyzed by electrophoresis (as for DNA sequencing). Fragments of DNA resulting from DNase cleavage within the region protected by protein binding are missing from the sample of DNA that was incubated with protein.

Transcription in Prokaryotes.

Cooper GM. The Cell: A Molecular Approach. 2nd edition. Sunderland (MA): Sinauer Associates; 2000.
Figure 8-54The DNA footprinting technique

(A) This technique requires a DNA molecule that has been labeled at one end (see Figure 8-24B). The protein shown binds tightly to a specific DNA sequence that is seven nucleotides long, thereby protecting these seven nucleotides from the cleaving agent. If the same reaction were performed without the DNA-binding protein, a complete ladder of bands would be seen on the gel (not shown). (B) An actual footprint used to determine the binding site for a human protein that stimulates the transcription of specific eucaryotic genes. These results locate the binding site about 60 nucleotides upstream from the start site for RNA synthesis. The cleaving agent was a small, iron-containing organic molecule that normally cuts at every phosphodiester bond with nearly equal frequency. (B, courtesy of Michele Sawadogo and Robert Roeder.)

Analyzing Protein Structure and Function.

Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002.
Figure 7-10The DNA footprinting technique

(A) This technique requires a DNA molecule that has been radioactively labeled at one end (see Figure7-6B). The protein shown binds tightly to a specific DNA sequence that is seven nucleotides long, thereby protecting these seven nucleotides from the cleaving agent. If the same reaction were carried out without the DNA-binding protein, a complete ladder of bands would be seen on the gel (not shown). (B) An actual footprint used to determine the binding site for a human protein that stimulates the transcription of specific eucaryotic genes. These results locate the binding site about 60 nucleotides upstream from the start site for RNA synthesis. The cleaving agent was a small, iron-containing organic molecule that normally cuts at every phosphodiester bond with nearly equal frequency. (B, courtesy of Michele Sawadogo and Robert Roeder.)

The Fragmentation, Separation, and Sequencing of DNA Molecules .

Alberts B, Bray D, Lewis J, et al. Molecular Biology of the Cell. 3rd edition. New York: Garland Science; 1994.
Figure 7-55Close-up view of the eve stripe 2 unit

The segment of the eve gene control region identified in the previous figure contains regulatory sequences, each of which binds one or another of four gene regulatory proteins. It is known from genetic experiments that these four regulatory proteins are responsible for the proper expression of eve in stripe 2. Flies that are deficient in the two gene activators Bicoid and Hunchback, for example, fail to efficiently express eve in stripe 2. In flies deficient in either of the two gene repressors, Giant and Krüppel, stripe 2 expands and covers an abnormally broad region of the embryo. The DNA-binding sites for these gene regulatory proteins were determined by cloning the genes encoding the proteins, overexpressing the proteins in E. coli, purifying them, and performing DNA-footprinting experiments as described in Chapter 8. The top diagram indicates that, in some cases, the binding sites for the gene regulatory proteins overlap and the proteins can compete for binding to the DNA. For example, binding of Krüppel and binding of Bicoid to the site at the far right are thought to be mutually exclusive.

How Genetic Switches Work.

Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002.