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Select item 14152801.

Madame Curie Bioscience Database [Internet].

Austin (TX): Landes Bioscience; 2000-2013.

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Select item 44036682.

StatPearls [Internet].

Treasure Island (FL): StatPearls Publishing; 2025 Jan-.

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Select item 18957883.

Molecular Biology of the Cell. 4th edition.

Alberts B, Johnson A, Lewis J, et al.

New York: Garland Science; 2002.

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Select item 53216724.

Essentials of Glycobiology [Internet]. 4th edition.

Varki A, Cummings RD, Esko JD, et al., editors.

Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022.

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Select item 54829305.

Glycoscience Protocols (GlycoPODv2) [Internet].

Nishihara S, Angata K, Aoki-Kinoshita KF, et al., editors.

Saitama (JP): Japan Consortium for Glycobiology and Glycotechnology; 2021-.

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Select item 16541066.

Immunobiology: The Immune System in Health and Disease. 5th edition.

Janeway CA Jr, Travers P, Walport M, et al.

New York: Garland Science; 2001.

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Select item 29026907.

Assay Guidance Manual [Internet].

Markossian S, Grossman A, Baskir H, et al., editors.

Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-.

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Select item 29069768.

TRP Channels.

Zhu MX, editor.

Boca Raton (FL): CRC Press/Taylor & Francis; 2011.

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Select item 32010449.

Personnel Needs and Training for Biomedical and Behavioral Research: 1985 Report.

Institute of Medicine (US) Committee on a Study of National Needs for Biomedical and Behavioral Research Personnel.

Washington (DC): National Academies Press (US); 1985.

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Select item 340129410.

Endotext [Internet].

Feingold KR, Adler RA, Ahmed SF, et al., editors.

South Dartmouth (MA): MDText.com, Inc.; 2000-.

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Select item 307786211.

Enterococci: From Commensals to Leading Causes of Drug Resistant Infection [Internet].

Gilmore MS, Clewell DB, Ike Y, et al., editors.

Boston: Massachusetts Eye and Ear Infirmary; 2014-.

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Select item 563221612.

National Cancer Institute’s Nanotechnology Characterization Laboratory Assay Cascade Protocols [Internet].

Bethesda (MD): National Cancer Institute (US); 2005 May 1-.

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Select item 255392413.

Neuroproteomics.

Alzate O, editor.

Boca Raton (FL): CRC Press/Taylor & Francis; 2010.

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Select item 140154014.

Indwelling Neural Implants: Strategies for Contending with the In Vivo Environment.

Reichert WM, editor.

Boca Raton (FL): CRC Press/Taylor & Francis; 2008.

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Select item 446506415.

Autoimmunity: From Bench to Bedside [Internet].

Anaya JM, Shoenfeld Y, Rojas-Villarraga A, et al., editors.

Bogota (Colombia): El Rosario University Press; 2013 Jul 18.

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Select item 166869016.

Holland-Frei Cancer Medicine. 6th edition.

Kufe DW, Pollock RE, Weichselbaum RR, et al., editors.

Hamilton (ON): BC Decker; 2003.

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Select item 323582417.

Chemical and Biological Terrorism: Research and Development to Improve Civilian Medical Response.

Institute of Medicine (US) Committee on R&D Needs for Improving Civilian Medical Response to Chemical and Biological Terrorism Incidents.

Washington (DC): National Academies Press (US); 1999.

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Select item 540176818.

Streptococcus pyogenes: Basic Biology to Clinical Manifestations [Internet]. 2nd edition.

Ferretti JJ, Stevens DL, Fischetti VA, editors.

Oklahoma City (OK): University of Oklahoma Health Sciences Center; 2022 Oct 8.

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Select item 458291619.

Some Naturally Occurring Substances: Food Items and Constituents, Heterocyclic Aromatic Amines and Mycotoxins.

IARC Working Group on the Evaluation of Carcinogenic Risks to Humans.

Lyon (FR): International Agency for Research on Cancer; 1993. (IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, No. 56.)

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Select item 518355620.

Biological Measures and Diagnostic Tools for Gulf War Illness: A Systematic Review [Internet].

Gean EG, Ayers CK, Winchell KA, et al.

Washington (DC): Department of Veterans Affairs (US); 2020 Sep.

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FIGURE 45.8

An example of the use of different immobilized plant lectins in serial lectin affinity chromatography of complex mixtures of glycopeptides. In this example, a mixture of glycopeptides is applied to a column of immobilized ConA, and the bound glycans are eluted with increasing concentrations of hapten sugars (α-methylglucose and then α-methylmannose) (arrows). The recovered glycans are then applied to a second set of columns containing immobilized LCA and bound glycans are eluted with the hapten α-methylmannose. This process is repeated over other immobilized lectins, such as MAL, and bound glycans are eluted with the hapten lactose. The glycans bound at each step are shown in the panels above the chromatographic peaks.
Symbol Key:

Antibodies and Lectins in Glycan Analysis.

Varki A, Cummings RD, Esko JD, et al., editors. Essentials of Glycobiology. 2nd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2009.
Figure 2:

Typical profile of the enzyme-linked immunosorbent assay (ELISA) analysis of ganglioside using a specific antibody.
The ganglioside samples are immobilized on the 96-well plate and detected using a specific antibody to ganglioside. Using known concentration of the authentic ganglioside sample, the concentration of the ganglioside in the sample is easily quantified.

Thin-layer chromatography-immunostaining method and enzyme-linked immunosorbent assay for detection of gangliosides by specific antibodies.

Nishihara S, Angata K, Aoki-Kinoshita KF, et al., editors. Glycoscience Protocols (GlycoPODv2) [Internet]. Saitama (JP): Japan Consortium for Glycobiology and Glycotechnology; 2021-.
Fig. 3

In vitro binding assays for nuclear import. (a) RanGTP-dependent release of Slx9 from Pse1 importin. Left panel: Workflow overview of the importin binding assay. POI = protein of interest. Right panel: GST–Pse1 complex formation with Slx9 dissociated in the RanGFP-dependent manner. GST–Pse1 (lanes 1–6) or GST alone (lanes 7–8) was immobilized on Glutathione Sepharose, washed with PBS-KMT and incubated with 4 μM Slx9 for 1 h at 4 °C (lanes 2–4 and 6). Pse1-Slx9 complex was subsequently incubated with 1.5 μM RanGTP under same conditions (lane 5). After washing with PBS-KMT, bound proteins were eluted in SDS sample buffer, separated by SDS-PAGE and visualized by Coomassie Blue staining and Western analyses using α-Slx9 antibodies. L = input. (b) RanGTP-independent release of eS26 from Kap123 importin. Immobilized GST-Kap123 on Glutathione Sepharose was washed and incubated with 4 μM eS26^FLAG for 1 h at 4 °C. GST-Kap123:eS26^FLAG complex was washed and incubated with either buffer alone (lane 2 and 8), 0.375 μM His₆-Ran^QLGTP (left panel lanes 3–6) or 0.375 μM His₆-Tsr2 (right panel lanes 9–12). Samples were withdrawn at the indicated time points and washed with PBS-KMT. Bound proteins were eluted in SDS sample buffer, separated by SDS-PAGE and visualized by Coomassie Blue staining and Western analyses using α-eS26 and α-RanGTP antibodies. L = input. GST-Kap123 is indicated by asterisk. (Adapted from Schütz et al. 2014)

Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport.

Entian KD, editor. Ribosome Biogenesis: Methods and Protocols [Internet]. New York (NY): Humana; 2022.
Figure 3

Enhanced Th2 cell differentiation accompanied with increased NF-κB activity and GATA3 expression in Shn-2-deficient CD4 T-cells. A) Naïve (CD44^low) CD4 T-cells from Shn-2-deficient (Shn-2−/−)x DO11.10 Tg mice were purified by cell sorting and stimulated with antigenic OVA peptide (Loh15: 0.1 μM) and irradiated BALB/c APCs for 5 days. Th2-skewed (IL-4 with anti-IL-12 mAb and anti-IFNγ mAb), Th1-skewed (IL-12 with anti-IL-4 mAb) and nonskewed (IL-2 with anti-IL-4 mAb, anti-IL-12 mAb and anti-IFNγ mAb) conditions were used. Intracellular staining was performed with FITC-conjugated anti-IFN-γ mAb and PE-conjugated anti-IL-4 mAb. Th2 cell differentiation is significantly increased in Shn-2-deficient CD4 T-cells. B) Splenic CD4 T-cells were cultured under Th2-skewed conditions for 16 hours. The cells were harvested and performed immunoblotting using anti-GATA3 or anti-Tubulin-α antibodies. Arbitrary densitometric units are shown under each band. GATA3 expression is increased in Shn-2-deficient cells. C) Splenic CD4 T-cells were incubated with medium alone overnight and then stimulated with immobilized anti-TCRαβ mAb for 3 hours. Nuclear extracts of the cultured cells were prepared and subjected to EMSAs with NF-κB probes. The supershift assays were performed with antibodies specific for NF-κB p50 and p65 subunit detection. Hyper-activation of NF-κB was observed in Shn-2-deficient CD4 T-cells. Reproduced from: Kimura et al., 2005. Originally published in The Journal of Experimental Medicine. doi:101084/jem.20040733.21

Memory Th1/Th2 Cell Generation Controlled by Schnurri-2.

Madame Curie Bioscience Database [Internet]. Austin (TX): Landes Bioscience; 2000-2013.