Figure 1.31. Two-photon excitation microscopy.

Figure 1.31Two-photon excitation microscopy

Simultaneous absorption of two photons is required to excite the fluorescent dye. This only occurs at the point in the specimen upon which the input light is focused, so fluorescent light is only emitted from the chosen depth of the specimen.

From: Tools of Cell Biology

Cover of The Cell
The Cell: A Molecular Approach. 2nd edition.
Cooper GM.
Sunderland (MA): Sinauer Associates; 2000.
Copyright © 2000, Geoffrey M Cooper.

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