Figure 11.36. Experimental demonstration of keratin function.

Figure 11.36Experimental demonstration of keratin function

A plasmid encoding a mutant keratin that interferes with the normal assembly of keratin filaments was microinjected into one pronucleus of a fertilized egg. Microinjected embryos were then transferred to a foster mother, and some of the offspring were found to have incorporated the mutant keratin gene into their genome. Expression of the mutant gene in these transgenic mice disrupted the keratin cytoskeleton of cells of the epidermis, resulting in severe skin blistering due to cell lysis following mild mechanical stress.

From: Intermediate Filaments

Cover of The Cell
The Cell: A Molecular Approach. 2nd edition.
Cooper GM.
Sunderland (MA): Sinauer Associates; 2000.
Copyright © 2000, Geoffrey M Cooper.

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