Figure 11 . – Progress curves for linear, rapid equilibrium inhibition (left) and nonlinear, time dependent inhibition (right).

Figure 11

– Progress curves for linear, rapid equilibrium inhibition (left) and nonlinear, time dependent inhibition (right). Nonlinear progress curves resulting from time dependent inhibition can be fit to the model shown above. The resulting fit will yield the initial velocity (vi), steady-state velocity (vs), and the rate constant for the interconversion between vi and vs (kobs), under the conditions tested. These values can be used to assess the true binding potency and modality.

From: Mechanism of Action Assays for Enzymes

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Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Brimacombe K, et al., editors.
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