Table 1:

Comparison of siRNA and shRNA-lentivirus

siRNAshRNA-lentivirus
Short-term target KD (< 1 week)Long-term target KD
Minimal library maintenanceSignificant library maintenance
Some cell types are not transfected efficientlyInfection is generally more effective than transfection, thus larger repertoire of cells can be used
Dosing to control cellular concentrationDifficult to control cellular concentration, though inducible system possible
Chemical modifications possibleStable target KD cell line can be generated.
More consistent quality of reagentTiter of shRNA-lentiviral particles can be more variable

From: Cell-Based RNAi Assay Development for HTS

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Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Brimacombe K, et al., editors.
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