Figure 6. . An example of a highly reproducible assay.

Figure 6.

An example of a highly reproducible assay. Pilot kinome screens conducted on separate occasions indicate highly reproducible assay conditions - a prerequisite for conducting an RNAi screen. Using siRNA sequences in pilot screens that are also represented in the large-scale campaign ensures that assay performance remained unchanged (compare “Ambion Druggable” to Kinome 1 and 2).

From: Cell-Based RNAi Assay Development for HTS

Cover of Assay Guidance Manual
Assay Guidance Manual [Internet].
Sittampalam GS, Coussens NP, Brimacombe K, et al., editors.
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