A demonstration of the Edinburgh pH-based arsenic biosensor with bromothymol blue as a pH indicator following static overnight incubation

FIGURE A5-1Demonstration of the Edinburgh pH-based arsenic biosensor, Escherichia coli JM109/pSB1A2-BBa_J33203 with bromothymol blue as pH indicator, following static overnight incubation

From left to right: arsenic-free control; 5, 10, 25, 50, and 100 ppb arsenic as sodium arsenate; and cell-free control with 100 ppb arsenate. Note the increasing size of the cell pellet in tubes with increasing arsenic concentrations. Color change occurs more rapidly in samples with increasing arsenic concentration (not shown).

SOURCE: C. French, unpublished.

From: A5, SYNTHETIC BIOLOGY AND THE ART OF BIOSENSOR DESIGN

Cover of The Science and Applications of Synthetic and Systems Biology
The Science and Applications of Synthetic and Systems Biology: Workshop Summary.
Institute of Medicine (US) Forum on Microbial Threats.
Washington (DC): National Academies Press (US); 2011.
Copyright © 2011, National Academy of Sciences.

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