Table 5.13Diagnostic accuracy of real-time polymerase chain reaction (PCR) versus culture in studies with a clinical gold standarda

StudyTest detailsSamplesReference standardPCR sensitivityPCR specificityBlood/CSF culture sensitivityBlood culture specificity
Bryant, 2004 68ctrA Taqman PCRBloodConsensus clinical diagnosis88%
n=24 cases
Hackett, 2002 69ctrA WB-Taqman PCRWhole bloodClinical diagnosis in the absence of other positive microbiology88%
n=98 cases
Carrol, 2000 70ctrA and IS1106 S-Taqman and PCR-ELISASerum/plasmaClinical diagnosis in the absence of other positive microbiology47%
n=166 cases
Van Gastel, 2007 71Duplex Taqman PCR targeting Neisseria meningitidis ctrA and Streptococcus pneumoniae plyCSFClinical features of meningitis plus positive CSF Gram stain, positive CSF or blood culture, or postive PCRMeningococcal meningitisn=23 cases
Pneumococcal meningitisn=14 cases

ctrA: N. meningitidis capsular transfer; CSF: cerebrospinal fluid

From: 5, Diagnosis in secondary care

Cover of Bacterial Meningitis and Meningococcal Septicaemia
Bacterial Meningitis and Meningococcal Septicaemia: Management of Bacterial Meningitis and Meningococcal Septicaemia in Children and Young People Younger than 16 Years in Primary and Secondary Care.
NICE Clinical Guidelines, No. 102.
National Collaborating Centre for Women's and Children's Health (UK).
London: RCOG Press; 2010.
Copyright © 2010, Royal College of Obstetricians and Gynaecologists.

No part of this publication may be reproduced, stored or transmitted in any form or by any means, without the prior written permission of the publisher or, in the case of reprographic reproduction, in accordance with the terms of licences issued by the Copyright Licensing Agency in the UK []. Enquiries concerning reproduction outside the terms stated here should be sent to the publisher at the UK address printed on this page.

The use of registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant laws and regulations and therefore for general use.

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.