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Metagenomics: Sequences from the Environment [Internet]. Bethesda (MD): National Center for Biotechnology Information (US); 2006.

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Metagenomics: Sequences from the Environment [Internet].

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Enhanced Biological Phosphorus Removal (EBPR) Sludge Community

Enhanced biological phosphorus removal (EBPR) is a treatment process in which microorganisms remove excessive inorganic phosphate from wastewater. However, the metabolic traits of this process are not well understood. Metagenomic analysis of two sludge samples allowed for the comparison of the microbial communities as well as the isolation of a near-complete genome of Acinetobacter phosphatis.


WGS studyEBPR sludge from Australia AATN00000000
EBPR sludge from USA AATO00000000
ContigsEBPR sludge from Australia AATN01000001-AATN01011188
EBPR sludge from USA AATO01000001-AATO01015866
ScaffoldsNo scaffolds available
Trace dataEBPR sludge from Australia 96,563 reads available from the Trace Archive
EBPR sludge from USA 127,953 reads available from the Trace Archive
16S ribosomal RNANo 16S rRNA sequences available

Approximately 98 and 78 Mbp of shotgun sequence data were obtained from the US and OZ (Australia) sludge, respectively.

Isolation Source

AustraliaActivated sludge from Thornside Sewage Treatment Plant in Queensland, Australia on August 18, 2004. The reactor was operated in four cycles of 6h per day, including 150 min anaerobic period, 180 min aerobic period, 30 min settling, and 5 min effluent withdrawing. The pH was kept at 7.0. The SBR was fed with propionate and a synthetic feed and nitrogen gas and air was bubbled through the liquid.
USAActivated sludge mixed liquor from the Nine Springs Wastewater Treatment Plant in Madison, WI, USA on July 3, 2004. The reactor was operated in four cycles of 6h per day, including 130 min anaerobic phase, 190 min aerobic phase, 30 min settling, and 10 min effluent withdrawing. The pH was in the range of 7.0-7.3. The sequencing batch reactor (SBR) was fed with acetate, casamino acids, and a mineral salts medium with sodium phosphate. The SBR was operating for 11 months at the time of sampling.

Genome Assembly



  1. Martin HG, Ivanova N, Kunin V, Warnecke F, Barry KW, McHardy AC, Yeates C, He S, Salamov AA, Szeto E, Dalin E, Putnam NH, Shapiro HJ, Pangilinan JL, Rigoutsos I, Kyrpides NC, Blackall LL, McMahon KD, Hugenholtz P. Metagenomic analysis of two enhanced biological phosphorus removal (EBPR) sludge communities. Nat Biotechnol. 2006 Oct;24(10):1263-1269. [PubMed: 16998472]


Sequence Analysis

  • BLAST against contigs from USA EBPR sludge.
  • BLAST against contigs from Australian EBPR sludge.
  • Genome Project ID: 17663

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