Table 2Reagents used for the uHTS experiments

ReagentVendor
U2OS (Human Osteosarcoma) cell line stably expressing GFP-tagged β-arrestin and over-expressing the GPR55 receptorCells from AP, scaled-up by SBCCG
MEM with L-glutamine, Pen-strep, 10% Fetal Bovine Serum and selection antibiotics - 100ug/ml G418 and 50ug/ml ZeocinInvitrogen
384-well plates, black with clear bottomGreiner
Agonist LysophosphatidylinositolAvanti Polar
Paraformaldehyde - FixativeACROS Organics
DAPI – Nuclei StainInvitrogen

From: Screening for Selective Ligands for GPR55 - Antagonists

Cover of Probe Reports from the NIH Molecular Libraries Program
Probe Reports from the NIH Molecular Libraries Program [Internet].

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