Figure 3. Activated versus dominant negative Rho GTPases.

Figure 3

Activated versus dominant negative Rho GTPases. The GAP-insensitive (G12V and Q61L) mutants are constitutively GTP-bound, and have therefore been used to characterize the different cellular functions of a variety of GTPases. By inhibiting Rho GEF function, dominant negative S17N versions have been key tools in studies assessing the contribution of Rho proteins to various extracellular signalling pathways. However, since they target multiple GEFs, the activities of S17N may have broader inhibitory actions on several GEFs, which then may block other Rho GTPases. Peptide fragments consisting of the GTP-dependent Rho binding regions (RBDs) of specific effectors can also act as select dominant negative versions of specific Rho proteins and have been used similarly to S17N.

From: Rho Family GTPases and Cellular Transformation

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