Figure 5. Localization of centrin in the mammalian retina and in photoreceptor fragments by indirect immunofluorescence.

Figure 5

Localization of centrin in the mammalian retina and in photoreceptor fragments by indirect immunofluorescence. A DAPI-staining of a longitudinal cryosection through the rat retina. Staining of nuclei DNA demonstrates the retinal layers: PC = layer of outer and inner segments of photoreceptor cells; ONL = outer nuclear layer where nuclei of photoreceptors are localized; OPL = outer plexiform layer; INL = inner nuclear layer; IPL = inner plexiform layer; GC = ganglion cell layer. B Indirect anti-centrin immunofluorescence in the cryosection through rat retina. Anti-centrin antibodies predominantly react within the photoreceptor cell layer at the joint between the inner and outer segment of the photoreceptors. In addition, indirect immunofluorescence is present in dot pairs in the inner nuclear layer and ganglion cell layer. C Higher magnification of immunofluorescent staining with antibodies against centrin in the inner nuclear layer of the section shown in figure B. Centrin is present in the centrioles of the centrosomes present in the perikaryon of retinal neurons. Note that as a rule one centriole of a single centrosome shows brighter anti-centrin immunofluorescence. D Schematic representation of a mammalian rod photoreceptor cell. The light-sensitive outer segment (OS) is linked via the nonmotile connecting cilium (CC) with the inner segment (IS) where the protein synthesis machinery is localized. N = nuclear region; S = synaptic region. Centrin localization is indicated by the green color of centrin in the PRC. E Indirect anti-centrin immunofluorescence of a photoreceptor fragment of the rat retina analysed by confocal laser scanning microscopy. RPE = rod pigment epithelium. The figure shows the a labeling of the connecting cilium and the basal body.

Bars in B = A: 20 μm; C: 7 μm, E: 2 μm.

From: Centrins, a Novel Group of Ca2+-Binding Proteins in Vertebrate Photoreceptor Cells

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