Figure 2. Two adjacent phenylalanines in the GR DBD are both required for a functional NES.

Figure 2

Two adjacent phenylalanines in the GR DBD are both required for a functional NES. The GFP reporter alone (no DBD) remains nuclear during the course of the experiment, while including the GR DBD (WT DBD) in the GFP reporter confers export. The FF⇒AA mutation has been shown to abolish the activity of the DBD NES.20,30 Mutation of either phenylalanine (F444A, F445A) also blocks DBD-mediated export, indicating that each is critical for the activity of the NES.

From: Calreticulin-Mediated Nuclear Protein Export

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