Figure 6. Left: Thin-layer chromatographic separation of substrate and product for the misaminoacylation of tRNAGln with glutamate by E.

Figure 6

Left: Thin-layer chromatographic separation of substrate and product for the misaminoacylation of tRNAGln with glutamate by E. coli GlnRS. The procedure is outlined in the text. Shown is a time course for the misaminoacylation from 0 to 300 seconds. Conditions are: 50 mM Tris (pH 7.4), 10 mM MgCl2, 5 mM ATP, 30 uM tRNA, 50 uM GlnRS and 500 mM glutamate. Right: Plot of the aminoacylation data shown at left. The first-order rate constant for aminoacylation under these conditions, at extremely high Glu concentration, is determined to be 0.03 sec-1.

From: Glutaminyl-tRNA Synthetases

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