Figure 3. Immunolocalization of EDA (A, C, E, G) and EDB (B, D, F, H) fibronectin in human oral mucosal wounds.

Figure 3

Immunolocalization of EDA (A, C, E, G) and EDB (B, D, F, H) fibronectin in human oral mucosal wounds. In 3-day wounds (A and B), EDA fibronectin was expressed under the epithelial cells migrating through the fibrin clot (A; arrowheads) but no expression of EDB fibronectin was observed (B). In 7-day wounds, the epithelium had completely covered the wound, and expression of EDA (C) and EDB (D) fibronectin was strongly up-regulated in the granulation tissue. The strongest expression of EDB fibronectin localized to the subepithelial granulation tissue (D). After 14 (E, F) and 28 (G, H) days, expression of EDA fibronectin was still strongly up-regulated (E, G) while the expression of EDB isoform (F, H) was gradually down-regulated in the granulation tissue. Even after 28 days, expression of both EDA (G) and EDB (H) fibronectin remained high as compared with normal connective tissue. For immunostaining, frozen tissue sections from human oral mucosal wounds were incubated with a monoclonal antibody against EDA (clone IST9; Accurate Chemical & Scientific Corp, Westbury, NY) or EDB fibronectin (BC1; kindly provided by Dr. Zardi, Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy). The EDA fibronectin was localized by an immunofluorescent secondary antibody and the EDB isoform by using the alkaline phosphatase (APAAP) method30 (Kindly performed by Dr. Kosmehl, Friedrich Schiller University, Jena, Germany). For the illustration, the digitized black and white images of EDB stainings were inverted by using Adobe Photoshop software. CT: connective tissue; E: epithelium; FC: fibrin clot; GT: granulation tissue. Large arrow: wound edge. Bar = 200 mm.

From: Keratinocyte Interactions with Fibronectin during Wound Healing

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