Figure 6. Double-staining of β-endorphin (red) with P-selectin (green) (A, B) and with PECAM-1 (green) (D, E) in noninflamed (A, D) and inflamed (B, E) subcutaneous paw tissue in the rat.

Figure 6

Double-staining of β-endorphin (red) with P-selectin (green) (A, B) and with PECAM-1 (green) (D, E) in noninflamed (A, D) and inflamed (B, E) subcutaneous paw tissue in the rat. C and F are controls: Preabsorption of β-endorphin antiserum with β-endorphin or the omission of the primary antibodies for P-selectin (C) or PECAM (F) revealed no significant immunoreactivity. Bar = 20 μm. Reprinted with permission from Mousa et al J Neuroimmunol 2000; 108:160–170. ©2000 Elsevier Science.

From: Morphological Correlates of Immune-Mediated Peripheral Opioid Analgesia

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