Figure 5. Calreticulin and calreticulin NH2-domain can bind to murine laminin.

Figure 5

Calreticulin and calreticulin NH2-domain can bind to murine laminin. A)Recombinant MBP-calreticulin, MBP-calreticulin NH2-domain, and MBP (0.16–1000 nM) were tested for binding to laminin in solid-phase assays. Laminin (25 μg/ml) was immobilized onto microtiter wells. Protein binding to laminin was measured by use of a rabbit anti-MBP calreticulin NH2-domain monospecific antiserum followed by a secondary affinity-purified goat anti-rabbit antiserum conjugated to alkaline phosphatase. The results reflect the mean absorbance at 405 nM. B)Laminin (0.25–60 μg/ml) was tested for binding to recombinant MBP-calreticulin, MBP-calreticulin NH2-domain or MBP immobilized onto microtiter wells. Comparable coating by the proteins was ensured. Laminin binding to the wells coated with MBP-calreticulin, MBP-calreticulin NH2-domain or MBP was detected by use of a rabbit anti-laminin antiserum and a secondary affinity-purified goat anti-rabbit serum conjugated to alkaline phosphatase.

From: Calreticulin and Tumor Suppression

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