FIGURE 3.2. A hybrid SERS label made from the Food and Drug Administration-approved dye indocyanine green (ICG) on gold nanoparticles and the application of this label inside living cells.

FIGURE 3.2A hybrid SERS label made from the Food and Drug Administration-approved dye indocyanine green (ICG) on gold nanoparticles and the application of this label inside living cells

(a) Examples of SERS spectra measured from single living cells incubated with the ICG-gold hybrid label at 830 nm excitation. Assignments of major bands are given below spectrum D. ICG bands are marked with an asterisk. (b) Spectral imaging of a SERS label in a living cell based on the SERS spectrum of ICG consisting of several narrow lines. For imaging the label, this offers the advantage that spectral correlation methods can be used to enhance the contrast between the label and the cellular background. A photomicrograph of the cell, indicating the mapped area, is shown for comparison. Scale bar: 20 microns.

SOURCE: Reprinted with permission from Kneipp, J., H. Kneipp, W.L. Rice, and K. Kneipp. 2005. Optical probes for biological applications based on surface-enhanced Raman scattering from indocyanine green on gold nanoparticles. Anal. Chem. 77:2381-2385. Copyright 2005 American Chemical Society.

From: 3, Imaging Techniques: State of the Art and Future Potential

Cover of Visualizing Chemistry
Visualizing Chemistry: The Progress and Promise of Advanced Chemical Imaging.
National Research Council (US) Committee on Revealing Chemistry through Advanced Chemical Imaging.
Washington (DC): National Academies Press (US); 2006.
Copyright © 2006, National Academy of Sciences.

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