Table 1Inducible Hsp60 mRNA and protein expression in monocytes

Inducing AgentsmRNAProtein
Heat shock (42°C)±±
Interferon-γ++++
Interferon-γ and TNFα++++++

THP-1, a human monocytic cell line, was incubated with 100U/ml of cytokine for 48 hrs. mRNA was assayed by Northern blot analysis, and protein analysis by Western blot using the ML-30 monoclonal antibody (Jindal et al, 1989; reviewed by Kiessling R et al, 1991).

From: Antigen Recognition by γδ T-Cells

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