Figure 2. MDM2 promotes p53 ubiquitination and degradation.

Figure 2

MDM2 promotes p53 ubiquitination and degradation. Saos-2 cells (p53 null) were transfected with DNAs encoding p53, wild-type MDM2, and a RING-finger mutant of MDM2 (C464A). P53 levels were then assessed by immunoblotting with an anti-p53 antibody. In some cases, cells were treated with a proteasome inhibitor for 6 hrs. prior to analysis. Wild-type MDM2 promotes p53 degradation while MDM2 C464A does not, indicating the RING-finger of MDM2 is required for p53 degradation. A ladder of high molecular weight p53 species is revealed when cells transfected with p53 and wild-type MDM2 are treated with the proteasome inhibitor. These bands are ubiquitinated forms of p53.

From: MDM2: RING Finger Protein and Regulator of p53

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