Figure 2. Expression of caspase-3, -7, -8, and -9 proenzymes in human cancer cells.

Figure 2

Expression of caspase-3, -7, -8, and -9 proenzymes in human cancer cells. A) Caspase proenzyme expression in E1A stable cells established in human breast cancer cell lines MCF-7, MDA-MB-231, and MDA-MB-453; and ovarian cancer cell line 2774. V: vector control; E: E1A stable cells. B) Procaspase enzyme expression in Rb wild-type breast cancer cells MCF-7, MDA-MB-231, MDA-MB-453; normal foreskin fibroblast cell SK-27; and osteosarcoma U2OS cells versus Rb-deficient cells (MDA-MB-468, BT-549, HBL-100, and Soas2). SK-27, U2OS, and Soas2 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA). Mouse monoclonal antibodies against human caspase-3 and -7 were from Transduction Laboratories (1:1000; C31720, Lexington, KY) and BD PharMingen (1:1000; 66871A, San Diego, CA), respectively. Rabbit polyclonal antibodies against human caspase-8 and -9 were from Santa Cruz Biotechnology (1:500; SC-7890/H-134, Santa Cruz, CA) and Cell Signaling Technology (1:500; #9502, Beverly, MA), respectively. A) Adapted with permission from reference 37 (Liao Y, Hung MC. Mol Cell Bio 2003; 23:6836).

From: Novel Approaches for Chemosensitization of Breast Cancer Cells: The E1A Story

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