Figure 2. MT are involved in a kinesin-dependent transport of N-cadherin-containing cargo.

Figure 2

MT are involved in a kinesin-dependent transport of N-cadherin-containing cargo. a) Schematic distribution of N-cadherin in the golgi, in secretory and endocytotic vesicles and at the plasma membrane. The N-cadherin-containing vesicles are moved along MT by kinesin motor proteins. b) N-cadherin/ GFP-expressing C2C12 myoblast was microinjected with X-rhodamine-labeled tubulin and the distribution of GFP and rhodamine-labeled probes was examined by time lapse microscopy. Images were captured every 10 s. for 10 min. and stacks of images were deconvolved using the Huygens System image restoration software. One deconvolved plan visualized using the Iview command of the Imaris software is shown. Respective voxel colocalization of N-cadherin/GFP with MT in the selected area (dashed line) obtained using the Imaris Colocalization module is shown in the yellow square. MT (in red) and the voxel colocalization (in yellow) are shown.

From: Cadherin-Mediated Cell-Cell Adhesion and the Microtubule Network

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