Figure 4. MDA-MB-435 cells transfected with maspin express the Mr 42,000 maspin protein.

Figure 4

MDA-MB-435 cells transfected with maspin express the Mr 42,000 maspin protein. The T6 and Tn-15 maspin transfectants and Nn-12 neo/sham transfected cells were plated on plastic or fibronectin for 1 hour. The cells were then scraped into RIPA buffer and equal amounts of protein loaded on 10% SDS-PAGE gels. After electrophoresis, the proteins were electrophoretically transferred to a polyvinylidene fluouride membrane and the resulting blot probed with a polyclonal anti-maspin antibody. After treating the blot with a peroxidase-conjugated secondary antibody, the maspin protein was located and identified using enhanced chemoluminescence (ECL).

From: Maspin Suppresses Breast Cancer Cell Invasiveness by Modulating Integrin Expression and Function

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