Figure 3. Identification of the cardiomyocyte T-Ag binding proteins.

Figure 3

Identification of the cardiomyocyte T-Ag binding proteins. AT-2 cardiomyocytes (a cell line derived from an atrial tumor which developed in transgenic mice expressing an MHC-promoted T-Ag transgene17) were grown in the presence of 35S-methionine to radiolabel the proteins. The cells were homogenized, and the resulting protein extract was reacted with PAb-419 (an anti-T-Ag monoclonal antibody), with PAb-240 (an IgG subtype-matched negative control monoclonal antibody which recognizes only mutant p53), or with PAb-421 (and anti-wild type p53 monoclonal antibody). Immune complex was collected on protein A-Sepharose beads, resolved on 10% denaturing polyacrylamide gels and visualized by autoradiography. Molecular weight standards are indicated on the left, and the T-Ag binding proteins are indicated on the right. [Adapted from ref. 18]

From: Regulation of Cardiomyocyte Proliferation and Apoptosis

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