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Contents
- GlycoPOD v2 Editorial Board
- Preface
- Purification and separation
- Glycoproteins
- Purification and analysis of free N-glycans in Saccharomyces cerevisiaeHiroto Hirayama and Tadashi Suzuki.Created: September 15, 2021; Last Revision: March 24, 2022.
- Synthesis of glycoproteins in vitroKodai Machida and Hiroaki Imataka.Created: September 27, 2021; Last Revision: March 15, 2022.
- Release of O-glycan by eliminative oximationAkihiko Kameyama.Created: October 1, 2021; Last Revision: March 24, 2022.
- Preparation of O-glycopeptides by GlycOCATCHDaisuke Takakura.Created: September 15, 2021; Last Revision: March 14, 2022.
- Preparation of glycopeptides by hydrophilic interaction chromatography (HILIC)Hiroyuki Kaji.Created: October 1, 2021; Last Revision: March 28, 2022.
- Preparation of glycopeptides by lectin affinity chromatography (LAC)Hiroyuki Kaji.Created: October 1, 2021; Last Revision: March 28, 2022.
- Preparation of glycopeptides by acetone precipitationDaisuke Takakura.Created: September 15, 2021; Last Revision: March 14, 2022.
- Purification and analysis of free N-glycans in Saccharomyces cerevisiae
- Glycosaminoglycans
- Preparation of glycosaminoglycansShuji Mizumoto and Shuhei Yamada.Created: October 6, 2021; Last Revision: March 30, 2022.
- Preparation of proteoglycans from tissuesHideto Watanabe.Created: October 2, 2021; Last Revision: March 18, 2022.
- Preparation of oligosaccharides from chondroitin sulfate with hyaluronidaseShuji Mizumoto.Created: October 16, 2021; Last Revision: March 18, 2022.
- Preparation of heparin/heparan sulfate oligosaccharidesShuhei Yamada.Created: October 6, 2021; Last Revision: March 30, 2022.
- Preparation of glycosaminoglycans
- Glycolipids
- Extraction of glycolipidsNozomu Okino and Makoto Ito.Created: September 28, 2021; Last Revision: March 21, 2022.
- Preparation of membrane raftsJin-ichi Inokuchi.Created: October 11, 2021; Last Revision: March 17, 2022.
- Thin-layer chromatography (TLC) of glycolipidsNozomu Okino and Makoto Ito.Created: September 29, 2021; Last Revision: March 22, 2022.
- TLC-blot (far-eastern blot) and its application to MS analysis of glycosphingolipidsNaoko Goto-Inoue and Takao Taki.Created: September 1, 2021; Last Revision: March 17, 2022.
- Preparation of dolichylpyrophosphoryl oligosaccharides from Saccharomyces cerevisiaeYoichiro Harada.Created: October 4, 2021; Last Revision: March 22, 2022.
- Extraction of glycolipids
- Oligosaccharides
- Large scale preparation of oligosaccharides from chicken eggsYasuhiro Kajihara.Created: November 11, 2021; Last Revision: March 22, 2022.
- Preparation of oligosaccharides from human milkJunko Amano.Created: October 4, 2021; Last Revision: March 17, 2022.
- Purification method for serum free glycansChengcheng Huang and Tadashi Suzuki.Created: November 2, 2021; Last Revision: March 24, 2022.
- Preparation of neutral free oligosaccharides from mammalian cultured cellsYoichiro Harada.Created: October 4, 2021; Last Revision: March 22, 2022.
- Large scale preparation of oligosaccharides from chicken eggs
- Lectins
- Production, purification and quality control of galectin-1Guillaume St-Pierre and Sachiko Sato.Created: October 23, 2021; Last Revision: March 30, 2022.
- Production, purification and quality control of galectin-3Guillaume St-Pierre and Sachiko Sato.Created: October 23, 2021; Last Revision: March 30, 2022.
- Preparation of recombinant galectin protein: expression, affinity purification, and removal of lipopolysaccharideTomoharu Takeuchi.Created: October 1, 2021; Last Revision: March 23, 2022.
- Isolation and determination of hepatic asialoglycoprotein receptorToshisuke Kawasaki and Hiromi Nakao.Created: October 23, 2021; Last Revision: March 25, 2022.
- Isolation of mannan-binding protein from human serum (plasma)Katsuaki Higashi, Motohiro Nonaka, Nobuko Kawasaki, and Toshisuke Kawasaki.Created: September 1, 2021; Last Revision: May 24, 2022.
- Isolation of pulmonary surfactant proteins from bronchoalveolar lavage fluidsShigeru Ariki, Chiaki Nishitani, and Yoshio Kuroki.Created: September 30, 2021; Last Revision: March 18, 2022.
- Purification and binding assay of human ficolin-2Misao Matsushita.Created: September 30, 2021; Last Revision: March 21, 2022.
- Isolation and binding assay of ER degradation-enhancing α-mannosidase-like proteins (EDEMs)Nobuko Hosokawa.Created: September 21, 2021; Last Revision: March 25, 2022.
- Production, purification and quality control of galectin-1
- Others
- Blue native polyacrylamide gel electrophoresisYoichiro Harada.Created: October 4, 2021; Last Revision: March 22, 2022.
- Preparation of mouse liver microsomeYoshimi Haga.Created: July 28, 2021; Last Revision: March 16, 2022.
- Enrichment of sulfated glycopeptides by sulfate emerging methodAkihiko Kameyama and Masaaki Toyoda.Created: October 1, 2021; Last Revision: March 24, 2022.
- Blue native polyacrylamide gel electrophoresis
- Glycoproteins
- Activity and functional analysis
- Enzymes: Hydrolases and lyases
- Endo-β-galactosidase digestionMichiko Fukuda, N and Tomoya Akama, O.Created: September 30, 2021; Last Revision: March 22, 2022.
- Enzyme assay of glycoproteins by endo-β-N-acetylglucosaminidasesDavid Martin C. de los Reyes, Kristina Mae Bienes, and Kaoru Takegawa.Created: September 14, 2021; Last Revision: March 25, 2022.
- A chemical probe for the detection of endo-β-N-acetylglucosaminidase activityNozomi Ishii and Ichiro Matsuo.Created: December 23, 2021; Last Revision: March 30, 2022.
- Enzyme assay for β-N-acetylhexosaminidaseHisashi Ashida and Kenji Yamamoto.Created: August 31, 2021; Last Revision: March 17, 2022.
- Enzyme assay for mammalian sialidasesTaeko Miyagi, Koji Yamamoto, and Kazuhiro Shiozaki.Created: October 7, 2021; Last Revision: March 22, 2022.
- Enzyme assay of endogenous activity of peptide-N-glycanase (PNGase)/N-Glycanase 1 (NGLY1) in cells and tissues using glycosylated cyclopeptide as a substrateHiroto Hirayama and Tadashi Suzuki.Created: September 15, 2021; Last Revision: March 25, 2022.
- Protein degradation assay for endoplasmic reticulum-associated degradation (ERAD) in mammalian cellsNobuko Hosokawa.Created: September 21, 2021; Last Revision: March 25, 2022.
- Enzymatic assay of nucleocytoplasmic O-linked β-N-acetylglucosaminidase (O-GlcNAcase)Kazuo Kamemura.Created: September 23, 2021; Last Revision: March 16, 2022.
- Detection of heparanase enzyme activityNobuaki Higashi.Created: November 12, 2021; Last Revision: March 26, 2022.
- Heparan sulfate/heparin digesting enzymes from bacteriaNaoko Nagai.Created: October 1, 2021; Last Update: November 23, 2021.
- Chondroitin sulfate/dermatan sulfate-digesting enzymes from bacteriaTakashi Kobayashi and Nobuo Sugiura.Created: August 19, 2021; Last Revision: March 17, 2022.
- Keratan sulfate-degrading enzymes from bacteriaKiwamu Yamagishi and Hideto Watanabe.Created: November 1, 2021; Last Revision: March 28, 2022.
- Bacterial glycosaminoglycan lyases to depolymerize hyaluronanShuhei Yamada.Created: October 5, 2021; Last Revision: March 30, 2022.
- Assay for dolichol-linked oligosaccharide: pyrophosphataseSheng-tao Li and Tadashi Suzuki.Created: August 30, 2021; Last Revision: March 15, 2022.
- Degradation of glycolipids by endoglycoceramidaseYohei Ishibashi and Makoto Ito.Created: September 29, 2021; Last Revision: March 21, 2022.
- Hydrolysis of ceramide by ceramidase (CDase) and measurement of CDase activityNozomu Okino and Makoto Ito.Created: September 28, 2021; Last Revision: March 18, 2022.
- Hydrolysis of glycosphingolipids by a sphingolipid ceramide N-deacylase (SCDase)Nozomu Okino and Makoto Ito.Created: September 28, 2021; Last Revision: March 18, 2022.
- Analysis of sphingolipid activator protein activity for sphingolipid hydrolasesJunko Matsuda, Kazuhito Tsuboi, and Azusa Yoneshige.Created: September 28, 2021; Last Revision: March 16, 2022.
- Endo-β-galactosidase digestion
- Enzymes: Transferases
- Assay of protein O-glucosyltransferase 1 (POGLUT1) glycosyltransferase activityKatsuki Sato, Tadanobu Takahashi, and Hideyuki Takeuchi.Created: October 15, 2021; Last Revision: March 19, 2022.
- Enzyme assay of glycolipid glycosyltransferases: Gb3/CD77 synthase (A4GALT)Koichi Furukawa, Keiko Furukawa, Robiul Bhuiyan, and Yuji Kondo.Created: January 8, 2022; Last Revision: March 22, 2022.
- Enzyme assay of yeast glycosyltransferases: α1,2-galactosyltransferaseTakehiko Yoko-o.Created: December 1, 2021; Last Revision: March 23, 2022.
- General methods for detection of enzyme reaction products of polypeptide N-acetylgalactosaminyltransferase, β1,3-glycosyltransferase, and β1,4-glycosyltransferasesAkira Togayachi, Takashi Sato, Tomomi Kubota, and Hisashi Narimatsu.Created: December 20, 2021; Last Revision: March 28, 2022.
- Enzyme assay of β1,3-glycosyltransferase familyAkira Togayachi.Created: December 20, 2021; Last Revision: March 28, 2022.
- Enzyme assay of protein O-mannosyltransferase (POMT1/2)Keiko Akasaka-Manya, Hiroshi Manya, and Tamao Endo.Created: October 27, 2021; Last Revision: March 23, 2022.
- Enzyme assay of N-acetylglucosaminyltransferase-III (GnT-III, MGAT3)Kento Maeda and Naoyuki Taniguchi.Created: October 1, 2021; Last Revision: March 23, 2022.
- Enzyme assay of N-acetylglucosaminyltransferase-IV (GnT-IVa,b, MGAT4A,B)Kento Maeda and Naoyuki Taniguchi.Created: October 1, 2021; Last Revision: March 23, 2022.
- Enzyme assay of N-acetylglucosaminyltransferase VI (GnT-VI, GnT-IVc, MGAT4C)Tomohiko Taguchi.Created: September 23, 2021; Last Revision: March 13, 2022.
- Enzyme assay of N-acetylglucosaminyltransferase-V (GnT-V, MGAT5)Kento Maeda and Naoyuki Taniguchi.Created: October 1, 2021; Last Revision: March 23, 2022.
- Enzyme assay of N-acetylglucosaminyltransferase IX/Vb (MGAT5B)Kei-ichiro Inamori, Kento Maeda, and Naoyuki Taniguchi.Created: October 1, 2021; Last Revision: March 15, 2022.
- Enzyme assay of FringeYuko Tashima.Created: November 15, 2021; Last Revision: March 23, 2022.
- Enzyme assay of O-GlcNAc transferaseSayad Md. Didarul Alam and Tetsuya Okajima.Created: August 14, 2021; Last Revision: March 25, 2022.
- Enzymatic assay of nucleocytoplasmic O-linked β-N-acetylglucosaminyltransferase (O-GlcNAc transferase, OGT)Kazuo Kamemura.Created: September 23, 2021; Last Revision: March 16, 2022.
- Enzyme assay of protein O-linked-mannose β1,2-N-acetylglucosaminyltransferase 1 and protein O-linked-mannose β1,4-N-acetylglucosaminyltransferase 2 (POMGNT1/2)Keiko Akasaka-Manya, Hiroshi Manya, and Tamao Endo.Created: January 25, 2022; Last Revision: March 24, 2022.
- Enzyme assay of polypeptide N-acetylgalactosaminyltransferase familyAkira Togayachi and Hisashi Narimatsu.Created: December 20, 2021; Last Revision: March 28, 2022.
- Enzyme assay of β1,3-N-acetylgalactosaminyltransferase 2 (B3GALNT2)Kiyohiko Angata.Created: December 20, 2021; Last Revision: March 26, 2022.
- Enzyme assay of glycolipid glycosyltransferases: GM2/GD2 synthase (β1,4-GalNAc transferase, B4GALNT1)Koichi Furukawa, Keiko Furukawa, Yuhsuke Ohmi, and Robiul Bhuiyan.Created: January 8, 2022; Last Revision: March 22, 2022.
- Enzyme assay of α1,3/4-fucosyltransferaseAkira Togayachi and Takashi Kudo.Created: December 20, 2021; Last Revision: March 25, 2022.
- Enzyme assay of fucosyltransferase 8 (FUT8)Hideyuki Ihara and Yoshitaka Ikeda.Created: October 1, 2021; Last Revision: March 22, 2022.
- Enzyme assay of O-fucosyltransferase 1 using Drosophila OFUT1Tetsuya Okajima.Created: November 13, 2021; Last Revision: March 25, 2022.
- Enzyme assay of sialyltransferases for glycoproteinsShou Takashima.Created: September 28, 2021; Last Revision: May 20, 2022.
- Enzyme assay of sialyltransferases for oligosaccharidesShou Takashima.Created: September 28, 2021; Last Revision: May 20, 2022.
- Enzyme assay of GM3 synthase (lactosylceramide α2,3-sialyltransferase, GM3S, ST3GAL5)Kei-ichiro Inamori and Jin-ichi Inokuchi.Created: November 11, 2021; Last Revision: May 23, 2022.
- Enzyme assay of glycolipid glycosyltransferases: GD3 synthase (α2,8-sialyltransferase, ST8SIAI)Koichi Furukawa, Keiko Furukawa, and Yuhsuke Ohmi.Created: January 8, 2022; Last Revision: March 22, 2022.
- Assay for enzymatic activity of polysialyltransferaseMasaya Hane and Chihiro Sato.Created: November 1, 2021; Last Revision: April 27, 2022.
- Enzyme assay of glucuronyltransferases for HNK-1 epitope (GlcAT-P, B3GAT1 and GlcAT-S, B3GAT2)Yasuhiko Kizuka, Jyoji Morise, and Shogo Oka.Created: September 7, 2021; Last Revision: March 14, 2022.
- Enzyme assay of glycosaminoglycan glycosyltransferases for chondroitin sulfateSatomi Nadanaka and Hiroshi Kitagawa.Created: October 13, 2021; Last Revision: March 20, 2022.
- Enzyme assay of glycosaminoglycan glycosyltransferases for heparan sulfateSatomi Nadanaka and Hiroshi Kitagawa.Created: October 13, 2021; Last Revision: March 20, 2022.
- Enzyme assay of hyaluronan synthaseNaoki Itano.Created: September 28, 2021; Last Revision: March 22, 2022.
- Enzyme assay of glycosaminoglycan glycosyltransferases for keratan sulfateAkira Seko.Created: September 7, 2021; Last Revision: March 23, 2022.
- Enzyme assay of xylosyltransferaseSatomi Nadanaka and Hiroshi Kitagawa.Created: October 13, 2021; Last Revision: March 20, 2022.
- Assay of glucoside α1,3-xylosyltransferase 1/2 (GXYLT1/2) and xyloside α1,3-xylosyltransferase 1 (XXYLT1) xylosyltransferase activityAmika Yoshioka, Yuuki Kurebayashi, and Hideyuki Takeuchi.Created: October 15, 2021; Last Revision: March 19, 2022.
- Enzyme assay of N-acetylglucosamine-6-O-sulfotransferasesKenji Uchimura.Created: September 26, 2021; Last Revision: March 18, 2022.
- Enzyme assay of sulfotransferase for HNK-1 epitope (HNK-1ST, CHST10)Yasuhiko Kizuka, Jyoji Morise, and Shogo Oka.Created: September 7, 2021; Last Revision: March 14, 2022.
- Enzyme assay of sulfotransferases for chondroitin/dermatanOsami Habuchi.Created: October 1, 2021; Last Revision: February 9, 2023.
- Enzyme assay of sulfotransferases for chondroitin sulfate/dermatan sulfateOsami Habuchi.Created: October 1, 2021; Last Revision: February 9, 2023.
- Enzyme assay of sulfotransferases for heparan sulfateHiroko Habuchi and Koji Kimata.Created: October 1, 2021; Last Revision: February 9, 2023.
- Enzyme assay of sulfotransferases for keratan sulfateAkira Seko.Created: September 7, 2021; Last Revision: March 23, 2022.
- Enzyme assay of cerebroside sulfotransferaseKoichi Honke.Created: October 19, 2021; Last Revision: March 15, 2022.
- Enzyme assay of protein O-mannose kinase (POMK)Hiroshi Manya and Tamao Endo.Created: January 25, 2022; Last Revision: March 24, 2022.
- Enzyme assay of fukutin and fukutin-related protein (ribitol phosphate transferase, FKTN and FKRP)Rieko Imae, Hiroshi Manya, and Tamao Endo.Created: November 10, 2021; Last Revision: March 24, 2022.
- Enzyme assay of ribitol xylosyltransferase 1 (RXYLT1)Shunsuke Hoshino, Hiroshi Manya, and Tamao Endo.Created: November 10, 2021; Last Revision: March 24, 2022.
- Enzyme assay of protein-O-mannosyl glycan glycosyltransferases (LARGE1/2)Kei-ichiro Inamori.Created: October 1, 2021; Last Revision: March 15, 2022.
- Assay of protein O-glucosyltransferase 1 (POGLUT1) glycosyltransferase activity
- Enzymes: Others
- Alteration of cellular sialic acid composition by transfection of cytidine monophospho-N-acetylneuraminic acid hydroxylaseYuko Naito-Matsui and Hiromu Takematsu.Created: September 24, 2021; Last Revision: March 15, 2022.
- Enzyme assay for CMP-Neu5Ac hydroxylase (CMAH)Hiromu Takematsu.Created: August 16, 2021; Last Revision: March 16, 2022.
- Enzyme assay of sialic acid 9-phosphate synthase (SPS)Di Wu and Ken Kitajima.Created: January 20, 2022; Last Revision: March 30, 2022.
- Enzyme assay of sialate-pyruvate lyase (SPL)Di Wu, Takahiro Nakagawa, and Ken Kitajima.Created: January 19, 2021; Last Revision: March 30, 2022.
- Enzyme assay of cytidine 5’-monophosphate sialic acid synthetase (CMAS)Di Wu and Ken Kitajima.Created: January 19, 2022; Last Revision: March 30, 2022.
- Alteration of cellular sialic acid composition by transfection of cytidine monophospho-N-acetylneuraminic acid hydroxylase
- Lectins
- Assay method for the antiproliferative activity of human galectin-9Aiko Itoh and Nozomu Nishi.Created: July 29, 2021; Last Revision: March 16, 2022.
- Expression and purification of recombinant human galectin-9Aiko Itoh and Nozomu Nishi.Created: July 29, 2021; Last Revision: March 22, 2022.
- Recombinant protein preparation and functional analysis of mannan-binding proteinMotohiro Nonaka and Toshisuke Kawasaki.Created: October 1, 2021; Last Revision: March 21, 2022.
- Assay method for the lectin activity of mannan-binding proteinKatsuaki Higashi, Motohiro Nonaka, Nobuko Kawasaki, and Toshisuke Kawasaki.Created: September 1, 2021; Last Revision: April 30, 2022.
- Selectin-binding analysis of tumor cellsKeiichiro Sakuma and Reiji Kannagi.Created: September 6, 2021; Last Revision: March 25, 2022.
- Modified Stamper-Woodruff cell-binding assayHiroro Kawashima.Created: January 20, 2022; Last Revision: March 22, 2022.
- Assay of glycan-mediated lymphocyte homingHiroto Kawashima.Created: January 20, 2022; Last Revision: March 22, 2022.
- Flow cytometric analysis of macrophage galactose-type C-type lectin 1 and 2 expression by the use of specific monoclonal antibodiesKaori Denda-Nagai, Ryuichi Murakami, and Tatsuro Irimura.Created: February 7, 2022; Last Revision: March 30, 2022.
- Binding assay of calreticulin using isothermal titration calorimetryIchiro Matsuo and Yoichi Takeda.Created: October 4, 2021; Last Revision: March 27, 2022.
- Expression and binding assay of endoplasmic reticulum-Golgi intermediate compartment protein 53 (ERGIC-53, LMAN1)Kazuo Yamamoto.Created: September 29, 2021; Last Revision: March 21, 2022.
- Expression and binding assay of vesicular integral membrane protein 36 (VIP36, LMAN2)Kazuo Yamamoto.Created: September 29, 2021; Last Revision: March 21, 2022.
- Analysis of intracellular lectins using reporter cell expressing lectin on its surfaceKazuo Yamamoto.Created: September 29, 2021; Last Revision: April 5, 2022.
- Recombinant sugar binding proteins and their functional analysis: Siglec-7Toshiyuki Yamaji and Yasuhiro Hashimoto.Created: September 17, 2021; Last Revision: March 14, 2022.
- Hemagglutination assay for the estimation of a carbohydrate-binding activity and determination of its carbohydrate-binding specificityGuillaume St-Pierre and Sachiko Sato.Created: October 17, 2021; Last Revision: March 30, 2022.
- Enzyme-linked immunosorbent assay (ELISA)-based method for determination of influenza virus-host receptor binding specificityYasuo Suzuki and Nongluk Sriwilaijaroen.Created: November 24, 2021; Last Revision: March 23, 2022.
- Assay method for the antiproliferative activity of human galectin-9
- Transporters
- Assay of nucleotide sugar transport activity (Golgi/ER transporter)Shoko Nishihara and Ken-ichi Nakayama.Created: August 16, 2021; Last Revision: April 30, 2022.
- Assay of 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transport activityShoko Nishihara.Created: August 11, 2021; Last Revision: April 30, 2022.
- Assay of nucleotide sugar transport activity (Golgi/ER transporter)
- Others
- Analyses of glucose sensor function of pancreatic islet cellsKazuaki Ohtsubo.Created: August 31, 2021; Last Revision: March 22, 2022.
- Enzyme-mediated activation of radical sources (EMARS)-based molecular interaction analysisNorihiro Kotani, Arisa Yamaguchi, and Koichi Honke.Created: August 11, 2021; Last Revision: March 28, 2022.
- Imaging of glycoforms by transmembrane Förster resonance energy transfer (FRET)Yoshimi Haga.Created: August 3, 2021; Last Update: September 6, 2021.
- Functional analyses of Mucin-1-mediated signaling and subsequent tumor progressionShungo Iwamoto, Naoki Itano, and Hiroshi Nakada.Created: September 25, 2021; Last Revision: March 30, 2022.
- Regulation of growth factor activity by heparin/heparan sulfate proteoglycanSatomi Nadanaka and Hiroshi Kitagawa.Created: October 26, 2021; Last Revision: March 23, 2022.
- Cell death/survival signal by ceramide and sphingosine-1-phosphateKazuyuki Kitatani, Makoto Taniguchi, and Toshiro Okazaki.Created: October 12, 2021; Last Revision: March 23, 2022.
- Inhibitors of glucosylceramide synthaseJin-ichi Inokuchi.Created: September 22, 2021; Last Revision: March 24, 2022.
- Induction of colitis and its analysesShinichiro Shinzaki and Eiji Miyoshi.Created: September 2, 2021; Last Revision: April 5, 2022.
- Rate of ER-associated degradation (ERAD) of misfolded proteins in Saccharomyces cerevisiaeAkira Hosomi.Created: October 1, 2021; Last Revision: March 18, 2022.
- Strategies of genome editing design for gene knockoutSeiya Mizuno and Hayate Suzuki.Created: September 29, 2021; Last Revision: March 14, 2022.
- Tetraploid rescue experimentMasahide Asano.Created: October 23, 2021; Last Revision: March 30, 2022.
- Analyses of glucose sensor function of pancreatic islet cells
- Enzymes: Hydrolases and lyases
- Glycan characterization
- Sample preparation for characterization
- Metabolic labeling of glycans by radioactive sugarsTakashi Ohkura.Created: September 28, 2021; Last Revision: February 3, 2022.
- Permethylation for glycan analysisAkihiko Kameyama.Created: October 1, 2021; Last Revision: March 24, 2022.
- Production of glycoproteins for structural analysesMasamichi Nagae and Yoshiki Yamaguchi.Created: September 28, 2021; Last Revision: January 19, 2022.
- Succinylation-Alcian blue staining for mucinsAkihiko Kameyama and Weijie Dong.Created: October 1, 2021; Last Revision: March 24, 2022.
- Conversion of pyridylamino sugar chains to corresponding reducing sugar chainsShin-ichi Nakakita.Created: August 24, 2021; Last Revision: March 21, 2022.
- Structural elucidation of oligosaccharides in glycosphingolipids by permethylation and methanolysisSaki Itonori.Created: September 9, 2021; Last Revision: May 2, 2022.
- Inhibition of N-glycan synthesis by tunicamycinMotohiro Nonaka.Created: October 1, 2021; Last Revision: March 24, 2022.
- DiI labeling experimentJyoji Morise and Shogo Oka.Created: September 7, 2021; Last Revision: March 14, 2022.
- Metabolic labeling of glycans by radioactive sugars
- Mass spectrometry (MS)
- Glycan analysis by simple manual MSn spectral matchingKen Hanzawa.Created: October 20, 2021; Last Revision: March 22, 2022.
- N-Glycan profiling by liquid chromatography-mass spectrometry (LC-MS)Miyako Nakano.Created: October 24, 2021; Last Update: December 21, 2021.
- Simultaneous determination of intracellular nucleotide sugarsKazuki Nakajima and Naoyuki Taniguchi.Created: October 20, 2021; Last Revision: March 23, 2022.
- Identification of N-glycosylated peptides using glycosylation site-specific stable isotope tagging and liquid chromatography/mass spectrometryHiroyuki Kaji.Created: October 1, 2021; Last Revision: March 26, 2022.
- O-Glycopeptide analysis by multi-tandem mass spectrometryMasaki Kurogochi.Created: August 19, 2021; Last Revision: March 14, 2022.
- O-Linked glycan analysis by β-elimination in the presence of pyrazolone analogue (BEP)Yasuro Shinohara, Hisatoshi Hanamatsu, and Jun-ichi Furukawa.Created: September 28, 2021; Last Revision: March 23, 2022.
- Sialic acid linkage-specific alkylamidation via ring-opening aminolysis (aminolysis-SALSA)Hisatoshi Hanamatsu, Takashi Nishikaze, and Jun-ichi Furukawa.Created: September 29, 2021; Last Update: December 23, 2021.
- Identification of intact glycopeptides by liquid chromatography/tandem mass spectrometry followed by database searchHiroyuki Kaji.Created: October 1, 2021; Last Revision: March 28, 2022.
- Ion mobility mass spectrometry of N-linked glycanKenji Hirose.Created: November 6, 2021; Last Revision: March 19, 2022.
- Structural characterization of glycosphingolipids by liquid chromatography-mass spectrometryAkemi Suzuki, Takahiro Nitta, Atit Silsirivanit, Naoto Oikawa, Junko Matsuda, and Jin-ichi Inokuchi.Created: September 25, 2021; Last Revision: February 27, 2022.
- Relative content analysis of glycosphingolipids by liquid chromatography-mass spectrometry with multiple reaction monitoringAkemi Suzuki, Takahiro Nitta, Atit Silsirivanit, and Jin-ichi Inokuchi.Created: September 25, 2021; Last Revision: February 27, 2022.
- Mass spectrometry imaging for glycosphingolipidsMd. Mahmudul Hasan, Mst. Afsana Mimi, Kenji Kikushima, Tomoaki Kahyo, and Mitsutoshi Setou.Created: September 29, 2021; Last Revision: March 18, 2022.
- Cellular glycome analysisJun-ichi Furukawa, Hisatoshi Hanamatsu, and Yasuro Shinohara.Created: September 28, 2021; Last Revision: March 22, 2022.
- Glycosphingolipid-glycan analysis of human whole serum/plasma based on an endoglycoceramidase digestionHisatoshi Hanamatsu and Jun-ichi Furukawa.Created: September 29, 2021; Last Revision: March 22, 2022.
- Determination of glycosaminoglycan attachment sites by LC-MS/MSFredrik Noborn, Nilsson Jonas, Nikpour Mahnaz, and Larson Göran.Created: October 15, 2021; Last Revision: March 22, 2022.
- Mass spectrometry analysis of sulfated glycosaminoglycan oligosaccharidesShuhei Yamada.Created: October 19, 2021; Last Revision: March 15, 2022.
- Glycan analysis by simple manual MSn spectral matching
- Nuclear magnetic resonance
- Determination of glycan structure by nuclear magnetic resonanceYoshiki Yamaguchi.Created: September 30, 2021; Last Revision: January 29, 2022.
- Determination of glycan structure by nuclear magnetic resonance
- Gas chromatography/liquid chromatography
- Carbohydrate analysis by gas-liquid chromatographyChihiro Sato.Created: September 11, 2021; Last Revision: January 26, 2022.
- Fluorescent labeling of glycans and high-performance liquid chromatography (HPLC) analysisShin-ichi Nakakita.Created: October 7, 2021; Last Revision: January 31, 2022.
- Assay of glucocerebrosidases using high-performance liquid chromatography and fluorescent substratesYasuhiro Hayashi.Created: October 6, 2021; Last Revision: May 11, 2022.
- Assay of glycosphingolipid synthases using high-performance liquid chromatography and fluorescent substratesYasuhiro Hayashi.Created: October 6, 2021; Last Revision: May 11, 2022.
- Simultaneous quantification of glucosylceramide and galactosylceramide by high-performance liquid chromatography using o-phtalaldehyde derivativesNozomu Okino and Makoto Ito.Created: October 11, 2021; Last Revision: March 21, 2022.
- High-performance liquid chromatography analysis of glycosaminoglycansShuhei Yamada and Hidenao Toyoda.Created: October 6, 2021; Last Revision: March 30, 2022.
- High-performance liquid chromatography analysis of hyaluronan unsaturated disaccharidesTomomi Izumikawa and Hidenao Toyoda.Created: November 1, 2021; Last Revision: February 11, 2023.
- High-performance liquid chromatography analysis of keratan sulfate oligosaccharidesTomomi Izumikawa and Hidenao Toyoda.Created: November 5, 2021; Last Revision: March 29, 2022.
- Multidimensional high-performance liquid chromatography-mapping method for N-linked glycan analysisHirokazu Yagi and Koichi Kato.Created: January 10, 2022; Last Revision: March 14, 2022.
- Two-mode analysis by high-performance liquid chromatography of p-amino benzoic acid ethyl ester-labeled monosaccharidesYuka Kobayashi, Hiroto Matsui, and Shunji Natsuka.Created: September 29, 2021; Last Revision: March 30, 2022.
- Standardization of glycan elution on high-performance liquid chromatographyKen Hanzawa.Created: January 18, 2022; Last Revision: March 24, 2022.
- Carbohydrate analysis by gas-liquid chromatography
- Antibody-based binding assays
- Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [1] ~ Characteristics and epitopes for anti-heparan sulfate antibodiesShuhei Yamada and Shuji Mizumoto.Created: October 1, 2021; Last Revision: March 30, 2022.
- Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [2] ~ Determination of the concentration of heparan sulfate using enzyme-linked immunosorbent assayShuhei Yamada.Created: October 5, 2021; Last Revision: March 22, 2022.
- Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [3] ~ Immunostaining using anti-heparan sulfate antibodyShuhei Yamada.Created: October 4, 2021; Last Revision: March 30, 2022.
- Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [4] ~ Detection of hyaluronan using biotinylated hyaluronan binding proteinsNaoki Itano.Created: September 28, 2021; Last Revision: March 22, 2022.
- Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [5] ~ enzyme-linked immunosorbent assay for hyaluronan quantificationShuhei Yamada, Shuji Mizumoto, and Naoki Itano.Created: October 4, 2021; Last Revision: March 30, 2022.
- Application of anti-glycosaminoglycan antibody: Immunostaining of keratan sulfate and sulfated N-acetyllactosamine oligosaccharidesHitomi Hoshino, Motohiro Kobayashi, and Kenji Uchimura.Created: September 9, 2021; Last Revision: March 18, 2022.
- Anti-chondroitin sulfate antibodies and their utility for immunostaining analysesTadahisa Mikami and Hiroshi Kitagawa.Created: September 22, 2021; Last Revision: January 12, 2022.
- Anti-chondroitin sulfate antibodies and their utility for enzyme-linked immunosorbent assay and western blottingTadahisa Mikami and Hiroshi Kitagawa.Created: October 2, 2021; Last Revision: March 15, 2022.
- Quantification of keratan sulfate in blood by enzyme-linked immunosorbent assay inhibition assayTomoya Akama, O.Created: September 30, 2021; Last Revision: March 23, 2022.
- Immunohistochemistry of mucin 21Kaori Denda-Nagai, Katrin B. Ishii-Schrade, Yuan Tian, Kyoko Okada, and Tatsuro Irimura.Created: October 19, 2021; Last Revision: March 30, 2022.
- Thin-layer chromatography-immunostaining method and enzyme-linked immunosorbent assay for detection of gangliosides by specific antibodiesShiori Go and Chihiro Sato.Created: September 27, 2021; Last Revision: March 24, 2022.
- Application of anti-glycosaminoglycan antibody and biotinylated hyaluronan binding protein (bHABP) [1] ~ Characteristics and epitopes for anti-heparan sulfate antibodies
- Lectin-based binding assays
- Binding assay of lectins and glycoproteins by surface plasmon resonanceYuka Kobayashi.Created: December 9, 2021; Last Revision: March 30, 2022.
- Lectin immunohistochemisty with Pholiota squarrosa lectin to evaluate core fucoseEiji Miyoshi and Yoshihiro Kamada.Created: August 24, 2021; Last Revision: April 1, 2022.
- Histochemical analysis of cell surface glycans by lectinMotohiro Nonaka.Created: August 20, 2021; Last Revision: March 21, 2022.
- Glycan profiling by lectin microarrayMasao Yamada and Jun Hirabayashi.Created: July 1, 2021; Last Revision: March 21, 2022.
- Tissue glycan analysis using lectin microarrayAtsushi Matsuda.Created: October 15, 2021; Last Revision: March 22, 2022.
- Lectin blottingMotohiro Nonaka and Toshisuke Kawasaki.Created: September 10, 2021; Last Revision: March 21, 2022.
- Binding assay of lectins and glycoproteins by surface plasmon resonance
- Other types of binding assays
- Thin-layer chromatography (TLC)-based assay for determination of influenza virus-host receptor binding specificityYasuo Suzuki and Nongluk Sriwilaijaroen.Created: November 24, 2021; Last Revision: March 22, 2022.
- Assay of the binding of bacteria to glycosphingolipidsShinichi Chisada, Naoyuki Matsunaga, and Makoto Ito.Created: September 30, 2021; Last Revision: March 20, 2022.
- Role of ganglioside GM1a in infection by human rotavirusesYasuo Suzuki and Chao-Tan Guo.Created: November 24, 2021; Last Revision: March 20, 2022.
- Surface plasmon resonance assay to measure binding specificity and relative affinity between noroviruses and synthetic histo-blood group antigensHaruko Shirato.Created: November 12, 2021; Last Revision: March 22, 2022.
- Thin-layer chromatography (TLC)-based assay for determination of influenza virus-host receptor binding specificity
- Other protocols for glycan characterization
- Partitioning of glycosylphosphatidylinositol (GPI)-anchored proteins with Triton X-114Morihisa Fujita, Yusuke Maeda, and Taroh Kinoshita.Created: September 17, 2021; Last Revision: March 25, 2022.
- Phosphatidylinositol-specific phospholipase C (PI-PLC) treatment of glycosylphosphatidylinositol (GPI)-anchored proteins on the cell surface of intact cellsMorihisa Fujita, Yusuke Maeda, and Taroh Kinoshita.Created: September 17, 2021; Last Revision: March 25, 2022.
- Pulse-chase transport analysis of decay accelerating factor (DAF) from the ER to the GolgiMorihisa Fujita, Yusuke Maeda, and Taroh Kinoshita.Created: September 17, 2021; Last Revision: March 25, 2022.
- Cellular synthesis of oligosaccharide using saccharide primerToshinori Sato.Created: October 15, 2021; Last Revision: March 22, 2022.
- Color reaction of carbohydratesShunji Natsuka.Created: September 17, 2021; Last Revision: March 24, 2022.
- Atomic force microscopic observation of reconstituted lipid bilayers containing glycosphingolipidsTeruhiko Matsubara and Toshinori Sato.Created: October 15, 2021; Last Revision: March 16, 2022.
- Partitioning of glycosylphosphatidylinositol (GPI)-anchored proteins with Triton X-114
- Sample preparation for characterization
- Chemical analysis
- Degradation
- Partial acetolysis for glycan linkage analysisShunji Natsuka and Shin-ichi Nakakita.Created: September 13, 2021; Last Revision: December 26, 2021.
- Smith degradation for glycan linkage analysisShunji Natsuka and Shin-ichi Nakakita.Created: September 14, 2021; Last Revision: January 26, 2022.
- Preparation of regioselectively 6-O-desulfated glycans by a non-destructive chemical method using silylating reagentsRyo Takano.Created: September 14, 2021; Last Revision: February 3, 2022.
- Hydrazinolysis using hydrazine monohydrate reducing sugar chainsShin-ichi Nakakita.Created: August 24, 2021; Last Revision: March 21, 2022.
- Partial acetolysis for glycan linkage analysis
- Labeling
- Release of N-glycans by hydrazinolysisShin-ichi Nakakita.Created: August 24, 2021; Last Revision: March 21, 2022.
- Specific labeling and chemical analysis of sialic acid and sialyloligo/polymersChihiro Sato.Created: September 11, 2021; Last Revision: March 24, 2022.
- Metabolic labeling of sphingolipidsMotohiro Tani, Hironobu Komori, and Makoto Ito.Created: September 15, 2021; Last Revision: May 21, 2022.
- Enzymatic fluorescent labeling of 6-gala (neogala) series glycosphingolipids-oligosaccharidesYohei Ishibashi and Makoto Ito.Created: October 5, 2021; Last Revision: March 21, 2022.
- Quantitative determination of ceramideNozomu Okino and Makoto Ito.Created: September 28, 2021; Last Revision: March 21, 2022.
- Quantitative determination of sphingomyelinNozomu Okino and Makoto Ito.Created: September 28, 2021; Last Revision: March 21, 2022.
- Release of N-glycans by hydrazinolysis
- Degradation
- Chemical synthesis
- Monosaccharide protection
- O-Acetylation using acetic anhydride in pyridineAkihiro Imamura.Created: October 6, 2021; Last Revision: March 17, 2022.
- De-O-acetylation using sodium methoxideAkihiro Imamura.Created: October 6, 2021; Last Revision: March 17, 2022.
- Benzylidene protection of diolShino Manabe.Created: September 30, 2021; Last Revision: April 1, 2022.
- Removal of benzylidene groupHidenori Tanaka.Created: October 6, 2021; Last Revision: April 5, 2022.
- Reductive opening of benzylidene groupHidenori Tanaka.Created: October 6, 2021; Last Revision: April 5, 2022.
- Benzylation of hydroxyl groups by Williamson reactionHiromune Ando.Created: October 6, 2021; Last Revision: March 19, 2022.
- Introduction of tert-butyldiphenylsilyl (TBDPS) groupHidenori Tanaka.Created: October 6, 2021; Last Revision: April 5, 2022.
- Introduction of 2,2,2-trichloroethoxycarbonyl (Troc) group at amino groupHidenori Tanaka.Created: October 6, 2021; Last Revision: April 5, 2022.
- O-Acetylation using acetic anhydride in pyridine
- Glycosyl donor preparation
- Synthesis of phenylthioglycosideAkihiro Imamura.Created: October 6, 2021; Last Revision: March 22, 2022.
- Conversion of thioglycosides into hemiacetalsHiromune Ando.Created: October 6, 2021; Last Revision: March 19, 2022.
- Synthesis of glycosyl imidate donorHiromune Ando.Created: October 6, 2021; Last Revision: March 19, 2022.
- Synthesis of glycosyl phosphate donorHiromune Ando.Created: October 6, 2021; Last Revision: March 19, 2022.
- Synthesis of glycosyl fluoride donorAkihiro Imamura.Created: October 6, 2021; Last Revision: March 17, 2022.
- Azidochrolination reaction of tri-O-acetyl-D-galactalKotaro Kanzaki and Osamu Kanie.Created: October 23, 2021; Last Revision: March 13, 2022.
- Synthesis of phenylthioglycoside
- Glycosidation
- Glycosidation using thioglycoside donorNaoko Komura.Created: October 6, 2021; Last Revision: March 19, 2022.
- Glycosidation using trichloroacetimidate donorNaoko Komura.Created: October 6, 2021; Last Revision: March 19, 2022.
- Glycosidation using phosphate donorNaoko Komura.Created: October 6, 2021; Last Revision: March 19, 2022.
- Glycosidation using fluoride donorNaoko Komura.Created: October 6, 2021; Last Revision: March 19, 2022.
- Glycosidation using thioglycoside donor
- Others
- Synthesis of the glycoprotein having an N-linked complex type oligosaccharideYasuhiro Kajihara and Ryo Okamoto.Created: November 11, 2021; Last Revision: March 22, 2022.
- Preparation of oxazoline derivatives from free saccharidesMasato Noguchi and Shin-ichiro Shoda.Created: August 30, 2021; Last Revision: March 18, 2022.
- Remodeling of immunoglobulin G (IgG) glycansTakashi Kinoshita and Shogo Iwamoto.Created: July 12, 2021; Last Revision: March 26, 2022.
- Synthesis of the glycoprotein having an N-linked complex type oligosaccharide
- Monosaccharide protection
- NLM CatalogRelated NLM Catalog Entries
- Glycoscience Protocols (GlycoPODv2)Glycoscience Protocols (GlycoPODv2)
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