FIGURE 3.2. Two-dimensional liquid chromatography on-line workflow.


Two-dimensional liquid chromatography on-line workflow. A fundamental difference in concept is applied to the on-line method. In this case instead of manually “loading” the first fractionation into the loading pump, the analytes are sent directly to a C18 enrichment column; after sample enrichment a valve is used to flush the analyte back into a nanobore column, where organic solvents are used to elute the sample directly into the mass spectrometer.

From: Chapter 3, Multidimensional Techniques in Protein Separations for Neuroproteomics

Cover of Neuroproteomics
Alzate O, editor.
Boca Raton (FL): CRC Press/Taylor & Francis; 2010.
Copyright © 2010 by Taylor and Francis Group, LLC.

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