FIGURE 15.7. 2D-DIGE results of individual terminal nerve branches of the rat femoral nerve, (a) and (b) Diagram of the two terminal nerve branches of the rat femoral nerve, showing the sensory cutaneous branch (continuing as the saphenous nerve and providing local cutaneous innervation) and the muscle branch to the quadriceps muscle.

FIGURE 15.7

2D-DIGE results of individual terminal nerve branches of the rat femoral nerve, (a) and (b) Diagram of the two terminal nerve branches of the rat femoral nerve, showing the sensory cutaneous branch (continuing as the saphenous nerve and providing local cutaneous innervation) and the muscle branch to the quadriceps muscle. Some animals received two ligations: one just distal to the parent femoral nerve bifurcation and one just proximal to the quadriceps muscle (as shown in a). Other animals received a single ligation just distal to the parent femoral nerve bifurcation (as shown in b). Individual muscle branch samples were harvested 7 days post-ligation. Muscle-derived proteins (indicated by red dots in b) include trophomorphic factors. The translucent tube-like structures within the nerve represent Schwann cell tubes that remain following nerve degeneration. DRG = dorsal root ganglion, MNs = motor neurons. (c) Nerve samples were labeled with CyDyes, combined, and run on the same 2D-DIGE gel. The combined image of the gel is shown (all three CyDyes), with an enlarged view to the right. Red spots are proteins expressed more highly in single-ligated nerve samples. Spots that appear white are expressed equally in all three samples. Graphical images of spot intensities from the single-ligated nerve sample (upper image) and double-ligated nerve sample (lower image) illustrate an example of expressions differences for a candidate trophomorphic mediator. Spots showing large expression differences were subsequently selected for protein identification.

From: Chapter 15, Applications of Proteomics to Nerve Regeneration Research

Cover of Neuroproteomics
Neuroproteomics.
Alzate O, editor.
Boca Raton (FL): CRC Press/Taylor & Francis; 2010.
Copyright © 2010 by Taylor and Francis Group, LLC.

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