FIGURE 8.8. CNG modulation and adaptation in ORCs.

FIGURE 8.8

CNG modulation and adaptation in ORCs. (A) Topological model and assembly of subunits of the olfactory CNG channel. Each transmembrane domain is indicated by a number, the pore loop is located between 5 and 6. The cyclic nucleotide-binding site (CNBD) is located in the C-terminal domain. Calmodulin-binding sites of the calcium-dependent “Baa type” are represented in black, whereas the calcium-independent “IQ-type” are in gray. (B) Native olfactory CNG channels are inhibited by Ca2+-calmodulin (CaM) in excised inside-out patches. A patch was exposed to 10 μM cAMP in a solution containing nominally 0 Ca2+. Then, the same patch was exposed to a solution containing, in addition to 10 μM cAMP, 1 μM calmodulin and 67 μM Ca2+ (in the presence of 1 mM NFA to block native Cl(Ca) current). The addition of Ca2+-CaM quickly inhibited the cAMP-gated current, which slowly recovered to its initial value after removal of Ca2+-CaM. (Modified from Pifferi S, Boccaccio A., and Menini A. FEBS Lett., 580, 2853–59, 2006a. With permission.)

From: Chapter 8, Signal Transduction in Vertebrate Olfactory Cilia

Cover of The Neurobiology of Olfaction
The Neurobiology of Olfaction.
Menini A, editor.
Boca Raton (FL): CRC Press/Taylor & Francis; 2010.
Copyright © 2010 by Taylor and Francis Group, LLC.

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