FIGURE 4.6. The CB1 antagonist, AM251, alters odor-evoked [Ca2+]j changes and electrical activity in individual ORNs of larval Xenopus laevis.

FIGURE 4.6

The CB1 antagonist, AM251, alters odor-evoked [Ca2+]j changes and electrical activity in individual ORNs of larval Xenopus laevis. A: Fluo4-AM-stained acute slice preparation of the OE (image acquired at rest; PC, principal cavity). The white ovals indicate the ORN somata of this slice that responded to a mixture of amino acids (scale bar 10 μm). B: [Ca2 + ]j transients of individual ORNs upon repeated applications of the amino acids are highly reproducible. The intraepithelial location of the four cells shown is indicated in A (scale bars: 10 s and ࢞F/F 100% [cells 1 and 2] and 10 s and ࢞F/F 50% [cells 3 and 4]). C: After the addition of AM251 to the bath solution, the amino acid-evoked ORN responses (black traces) were modulated (light gray traces). After 12 min of drug washout, the amino acid-induced [Ca2 + ]j transients recovered completely (dark gray traces) (scale bars: 10 s and AF/F 100% [cells 1 and 2] and 10 s and AF/F 50% [cells 3 and 4]). D: Amino acid-induced action potential-associated currents of an individual ORN (scale bars: 2 s and 20 pA [D1-D5]). The modulatory effect of AM251 on the action potential-associated currents depends on the wash-in time of the antagonist (A2-A4). Recovery after 20 min of drug washout (A5). The gray-shaded area indicates the time window of the original response. (Modified from Czesnik, D., Schild, D., Kuduz, J., and Manzin, I., Proc. Natl. Acad. Sci. USA., 104, 2967–72, 2007.)

From: Chapter 4, Olfactory Coding in Larvae of the African Clawed Frog Xenopus laevis

Cover of The Neurobiology of Olfaction
The Neurobiology of Olfaction.
Menini A, editor.
Boca Raton (FL): CRC Press/Taylor & Francis; 2010.
Copyright © 2010 by Taylor and Francis Group, LLC.

NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.