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National Toxicology Program. NTP Technical Report on the Toxicity Studies of o-Chloropyridine (CASRN 109-09-1) Administered Dermally and in Drinking Water to F344/N Rats and B6C3F1/N Mice: Toxicity Report 83 [Internet]. Research Triangle Park (NC): National Toxicology Program; 2017 Feb.

Cover of NTP Technical Report on the Toxicity Studies of o-Chloropyridine (CASRN 109-09-1) Administered Dermally and in Drinking Water to F344/N Rats and B6C3F1/N Mice

NTP Technical Report on the Toxicity Studies of o-Chloropyridine (CASRN 109-09-1) Administered Dermally and in Drinking Water to F344/N Rats and B6C3F1/N Mice: Toxicity Report 83 [Internet].

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Appendix HIngredients, Nutrient Composition, and Contaminant Levels in NTP-2000 Rat and Mouse Ration

Table H-1Ingredients of NTP-2000 Rat and Mouse Ration

IngredientsPercent by Weight
Ground hard winter wheat22.26
Ground #2 yellow shelled corn22.18
Wheat middlings15.0
Oat hulls8.5
Alfalfa meal (dehydrated, 17% protein)7.5
Purified cellulose5.5
Soybean meal (49% protein)5.0
Fish meal (60% protein)4.0
Corn oil (without preservatives)3.0
Soy oil (without preservatives)3.0
Dried brewer’s yeast1.0
Calcium carbonate (USP)0.9
Vitamin premixa0.5
Mineral premixb0.5
Calcium phosphate, dibasic (USP)0.4
Sodium chloride0.3
Choline chloride (70% choline)0.26
Methionine0.2
a

Wheat middlings as carrier.

b

Calcium carbonate as carrier.

Table H-2Vitamins and Minerals in NTP-2000 Rat and Mouse Rationa

AmountSource
Vitamins
A4,000 IUStabilized vitamin A palmitate or acetate
D1,000 IUD-activated animal sterol
K1.0 mgMenadione sodium bisulfite complex
α-Tocopheryl acetate100 IU–
Niacin23 mg–
Folic acid1.1 mg–
d-Pantothenic acid10 mgd-Calcium pantothenate
Riboflavin3.3 mg–
Thiamine4 mgThiamine mononitrate
B125 µg–
Pyridoxine6.3 mgPyridoxine hydrochloride
Biotin0.2 mgd-Biotin
Minerals
Magnesium514 mgMagnesium oxide
Iron35 mgIron sulfate
Zinc12 mgZinc oxide
Manganese10 mgManganese oxide
Copper2.0 mgCopper sulfate
Iodine0.2 mgCalcium iodate
Chromium0.2 mgChromium acetate
a

Per kg of finished product.

Table H-3Nutrient Composition of NTP-2000 Rat and Mouse Ration

NutrientMean ± Standard DeviationRangeNumber of Samples
Protein (% by weight)15.215.21
Crude fat (% by weight)8.08.01
Crude fiber (% by weight)9439.41
Ash (% by weight)5.35.31
Amino Acids (% of total diet)
Arginine0.783 ± 0.0700.670–0.97022
Cystine0.220 ± 0.0240.150–0.25022
Glycine0.701 ± 0.0410.620–0.80022
Histidine0.352 ± 0.0770.270–0.68022
Isoleucine0.546 ± 0.0440.430–0.66022
Leucine1.095 ± 0.0670.960–1.24022
Lysine0.711 ± 0.1140.310–0.86022
Methionine0.409 ± 0.0460.260–0.49022
Phenylalanine0.628 ± 0.0400.540–0.72022
Threonine0.505 ± 0.0430.430–0.61022
Tryptophan0.150 ± 0.0280.110–0.20022
Tyrosine0.401 ± 0.0610.280–0.54022
Valine0.665 ± 0.0430.550–0.73022
Essential Fatty Acids (% of total diet)
Linoleic3.95 ± 0.2593.49–4.5522
Linolenic0.30 ± 0.0320.21–0.3522
Vitamins
Vitamin A (IU/kg)6,9206,9201
Vitamin D (IU/kg)1,000 a––
α-Tocopherol (ppm)80.6 ± 22.0327.0–124.022
Thiamine (ppm)b8.88.81
Riboflavin (ppm)7.6 ± 2.894.20–17.5022
Niacin (ppm)78.9 ± 9.0866.4–98.222
Pantothenic acid (ppm)26.9 ± 12.6317.4–81.022
Pyridoxine (ppm)b9.54 ± 1.996.44–13.722
Folic acid (ppm)1.62 ± 0.481.15–3.2722
Biotin (ppm)0.32 ± 0.100.20–0.70422
Vitamin B12 (ppb)53.6 ± 39.618.3–174.022
Choline (ppm)b2,846 ± 4851,820–3,79022
Minerals
Calcium (%)0.9590.9591
Phosphorus (%)0.5890.5891
Potassium (%)0.666 ± 0.0300.626–0.73322
Chloride (%)0.386 ± 0.0390.300–0.47422
Sodium (%)0.189 ± 0.0160.160–0.22222
Magnesium (%)0.216 ± 0.0620.185–0.49022
Sulfur (%)0.170 ± 0.0290.116–0.20914
Iron (ppm)186 ± 39.2135–31122
Manganese (ppm)51.4 ± 10.2821.0–73.122
Zinc (ppm)53.4 ± 8.4643.3–78.522
Copper (ppm)7.01 ± 2.5623.21–16.322
Iodine (ppm)0.503 ± 0.2060.158–0.97222
Chromium (ppm)0.694 ± 0.2760.330–1.38022
Cobalt (ppm)0.256 ± 0.1640.098–0.86422
a

From formulation.

b

As hydrochloride (thiamine and pyridoxine) or chloride (choline).

Table H-4Contaminant Levels in NTP-2000 Rat and Mouse Rationa

MeanbRangeNumber of Samples
Contaminants
Arsenic (ppm)0.500.501
Cadmium (ppm)0.080.081
Lead (ppm)0.050.051
Mercury (ppm)<0.02–1
Selenium (ppm)0.160161
Aflatoxins (ppb)<5.00–1
Nitrate nitrogen (ppm)c17.717.71
Nitrite nitrogen (ppm)c<0.61–1
BHA (ppm)d<1.0–1
BHT (ppm)d<1.0–1
Aerobic plate count (CFU/g)10101
Coliform (MPN/g)3.03.01
Escherichia coli (MPN/g)<10–1
Salmonella (MPN/g)Negative–1
Total nitrosoamines (ppb)e3.53.51
N-Nitrosodimethylamine (ppb)e1.81.81
N-Nitrosopyrrolidine (ppb)e1.71.71
Pesticides (ppm)
α-BHC<0.01–1
β-BHC<0.02–1
γ-BHC<0.01–1
δ-BHC<0.01–1
Heptachlor<0.01–1
Aldrin<0.01–1
Heptachlor epoxide<0.01–
DDE<0.01–1
DDD<0.01–1
DDT<0.01–1
HCB<0.01–1
Mirex<0.01–1
Methoxychlor<0.05–1
Dieldrin<0.01–1
Endrin<0.01–1
Telodrin<0.01–1
Chlordane<0.05–1
Toxaphene<0.10–1
Estimated PCBs<0.20–1
Ronnel<0.01–1
Ethion<0.02–1
Trithion<0.05–1
Diazinon<0.10–1
Methyl chlorpyrifos0.1750.1751
Methyl parathion<0.02–1
Ethyl parathion<0.02–1
Malathion0.5890.5891
Endosulfan I<0.01–1
Endosulfan II<0.01–1
Endosulfan sulfate<0.03–1
a

All samples were irradiated. CFU = colony-forming units; MPN = most probable number; BHC = hexachlorocyclohexane or benzene hexachloride.

b

For values less than the limit of detection, the detection limit is given as the mean.

c

Sources of contamination: alfalfa, grains, and fish meal.

d

Sources of contamination: soy oil and fish meal.

e

All values were corrected for percent recovery.

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This is a work of the US government and distributed under the terms of the Public Domain

Bookshelf ID: NBK551148
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