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Ariel P. UltraMicroscope II – A User Guide [Internet]. Chapel Hill (NC): University of North Carolina at Chapel Hill, University Libraries; 2018 Sep 20-.
Members of labs or core facilities who already have, or want to install, a LaVision BioTec UltraMicroscope II light sheet system (Figure 1.1).
LaVision BioTec UltraMicroscope II, zoom optics version.
I’ve worked in two cores (at Rockefeller University and the University of North Carolina at Chapel Hill) where this microscope was implemented. Over the past four years I’ve gained hundreds of hours of experience with the system using samples from more than 40 labs from a dozen institutions. Along the way, I’ve made a lot of mistakes, learned from them, and improved our procedures. I’ve also benefitted enormously from many discussions with colleagues facing the same challenges. Finally, I don’t work for LaVision BioTec and am not interested in selling you a microscope; I’m trying to present an honest assessment of the benefits and pitfalls of this instrument, and some best practices, based on my experience as a user.
To help other users avoid reinventing the wheel. I believe this technology is mature enough that we should all share a baseline of best practices that is easily accessible to anyone with this microscope. We can (and should!) continue to improve our procedures, as well as quibble about many details, but I strongly believe all users should have a foundation of knowledge on which to build their own implementation of the system.
A guide for tissue clearing and labeling, a detailed manual for using the software, a recipe for the single best way to image samples with this microscope, or a guide to image analysis. For clearing and labeling, there is prolific literature to consult. For the ImSpector Pro software, LaVision BioTec provides a manual, as well as training during installation. For the imaging, there is no “best” way, only a multitude of options with different tradeoffs, which I will discuss at length. How to analyze images is a vast topic in which my expertise barely scratches the surface.
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